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PMID: 7568093 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Targeted disruption of vinculin genes in F9 and embryonic stem cells changes cell morphology, adhesion, and locomotion.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 20 ·1995-09-26 ·Pages 9161-5

Coll JL, Ben-Ze'ev A, Ezzell RM, Rodríguez Fernández JL, Baribault H, Oshima RG, Adamson ED

Abstract

Vinculin, a major constituent of focal adhesions and zonula adherens junctions, is thought to be involved in linking the microfilaments to areas of cell-substrate and cell-cell contacts. To test the role of vinculin in cell adhesion and motility, we used homologous recombination to generate F9 embryonal carcinoma and embryonic stem cell clones homozygous for a disrupted vinculin gene. When compared to wild-type cells, vinculin-mutant cells displayed a rounder morphology and a reduced ability to adhere and spread on plastic or fibronectin. Decreased adhesion of the mutant cells was associated with a reduction in lamellipodial extensions, as observed by time-lapse video microscopy. The locomotive activities of control F9 and the vinculin-null cells were compared in two assays. Loss of vinculin resulted in a 2.4-fold increase in cell motility. These results demonstrate an important role for vinculin in determining cell shape, adhesion, surface protrusive activity, and cell locomotion.

MeSH Terms
Actins/analysis Animals Cell Adhesion Cell Differentiation Cell Line Cell Movement Clone Cells Embryo, Mammalian Endoderm Fibronectins Humans Kinetics Mice Molecular Sequence Data Restriction Mapping Stem Cells/cytology,physiology Teratocarcinoma Transfection Tumor Cells, Cultured Vinculin/analysis,biosynthesis,genetics
Chemicals
Actins Fibronectins Vinculin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Coll J L
La Jolla Cancer Research Foundation, CA 92037, USA.
Ben-Ze'ev A
Ezzell R M
Rodríguez Fernández J L
Baribault H
Oshima R G
Adamson E D
References (35)
35 references, click to expand
  1. Targeted deletion of beta 1 integrins in F9 embryonal carcinoma cells affects morphological differentiation but not tissue-specific gene expression.
    J Cell Biol. 1993 Dec;123(6 Pt 1):1607-20 PMID: 7504677
  2. Suppression of vinculin expression by antisense transfection confers changes in cell morphology, motility, and anchorage-dependent growth of 3T3 cells.
    J Cell Biol. 1993 Sep;122(6):1285-94 PMID: 8376463
  3. Vinculin-deficient PC12 cell lines extend unstable lamellipodia and filopodia and have a reduced rate of neurite outgrowth.
    J Cell Biol. 1994 Nov;127(4):1071-84 PMID: 7962069
  4. Focal adhesion formation by F9 embryonal carcinoma cells after vinculin gene disruption.
    J Cell Sci. 1995 Jun;108 ( Pt 6):2253-60 PMID: 7673345
  5. cDNA-derived sequence of chicken embryo vinculin.
    Proc Natl Acad Sci U S A. 1988 Nov;85(22):8535-9 PMID: 3141928
  6. Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton.
    Annu Rev Cell Biol. 1988;4:487-525 PMID: 3058164
  7. The basal component of the nematode dense-body is vinculin.
    J Biol Chem. 1989 Jun 15;264(17):10177-85 PMID: 2498337
  8. Enhancement of vinculin synthesis by migrating stratified squamous epithelium.
    J Cell Biol. 1989 Aug;109(2):571-6 PMID: 2503524
  9. Identification of a talin binding site in the cytoskeletal protein vinculin.
    J Cell Biol. 1989 Dec;109(6 Pt 1):2917-27 PMID: 2512301
  10. Regulation of fibronectin, integrin and cytoskeleton expression in differentiating adipocytes: inhibition by extracellular matrix and polylysine.
    Differentiation. 1989 Dec;42(2):65-74 PMID: 2633939
  11. Complete sequence of human vinculin and assignment of the gene to chromosome 10.
    Proc Natl Acad Sci U S A. 1990 Aug;87(15):5667-71 PMID: 2116004
  12. An interaction between alpha-actinin and the beta 1 integrin subunit in vitro.
    J Cell Biol. 1990 Aug;111(2):721-9 PMID: 2116421
  13. Paxillin: a new vinculin-binding protein present in focal adhesions.
    J Cell Biol. 1990 Sep;111(3):1059-68 PMID: 2118142
  14. Transient induction of vinculin gene expression in 3T3 fibroblasts stimulated by serum-growth factors.
    Cell Regul. 1990 Aug;1(9):621-36 PMID: 2127699
  15. The induction of differentiation in teratocarcinoma stem cells by retinoic acid.
    Cell. 1978 Oct;15(2):393-403 PMID: 214238
  16. Developmental expression of murine extra-embryonic endodermal cytoskeletal proteins.
    J Biol Chem. 1982 Apr 10;257(7):3414-21 PMID: 6174520
  17. Formation of vinculin plaques precedes other cytoskeletal changes during retinoic acid-induced teratocarcinoma cell differentiation.
    Exp Cell Res. 1983 Mar;144(1):191-7 PMID: 6188622
  18. Platelet-derived growth factor-induced alterations in vinculin and actin distribution in BALB/c-3T3 cells.
    J Cell Biol. 1985 Apr;100(4):1031-40 PMID: 3920222
  19. Cell contact- and shape-dependent regulation of vinculin synthesis in cultured fibroblasts.
    Nature. 1986 Feb 27-Mar 5;319(6056):787-91 PMID: 3081814
  20. Evidence for the existence of an early common biochemical pathway in the differentiation of F9 cells into visceral or parietal endoderm: modulation by cyclic AMP.
    Dev Biol. 1986 Apr;114(2):492-503 PMID: 2420663
  21. Interaction of plasma membrane fibronectin receptor with talin--a transmembrane linkage.
    Nature. 1986 Apr 10-16;320(6062):531-3 PMID: 2938015
  22. Changes in the distribution of vinculin during preimplantation mouse development.
    Differentiation. 1986;32(2):125-34 PMID: 3098616
  23. In vitro regulation of granulosa cell differentiation. Involvement of cytoskeletal protein expression.
    J Biol Chem. 1987 Apr 15;262(11):5366-76 PMID: 3104332
  24. Contact-dependent regulation of vinculin expression in cultured fibroblasts: a study with vinculin-specific cDNA probes.
    EMBO J. 1987 Oct;6(10):2897-905 PMID: 3121302
  25. Epidermal growth factor activation of vinculin and beta 1-integrin gene transcription in quiescent Swiss 3T3 cells. Regulation through a protein kinase C-independent pathway.
    J Biol Chem. 1991 Jun 25;266(18):12008-14 PMID: 1711046
  26. Vinculin is essential for muscle function in the nematode.
    J Cell Biol. 1991 Aug;114(4):715-24 PMID: 1907975
  27. The cytoplasmic domain of adherens-type junctions.
    Cell Motil Cytoskeleton. 1991;20(1):1-6 PMID: 1756576
  28. Production of homozygous mutant ES cells with a single targeting construct.
    Mol Cell Biol. 1992 May;12(5):2391-5 PMID: 1569957
  29. Overexpression of vinculin suppresses cell motility in BALB/c 3T3 cells.
    Cell Motil Cytoskeleton. 1992;22(2):127-34 PMID: 1633623
  30. Regulation of adherens junction protein expression in growth-activated 3T3 cells and in regenerating liver.
    Exp Cell Res. 1992 Oct;202(2):477-86 PMID: 1383015
  31. Suppression of tumorigenicity in transformed cells after transfection with vinculin cDNA.
    J Cell Biol. 1992 Oct;119(2):427-38 PMID: 1400584
  32. Targeted disruption of the mouse transforming growth factor-beta 1 gene results in multifocal inflammatory disease.
    Nature. 1992 Oct 22;359(6397):693-9 PMID: 1436033
  33. Expression of chicken vinculin complements the adhesion-defective phenotype of a mutant mouse F9 embryonal carcinoma cell.
    J Cell Biol. 1993 May;121(4):909-21 PMID: 8491782
  34. Maximal migration of human smooth muscle cells on fibronectin and type IV collagen occurs at an intermediate attachment strength.
    J Cell Biol. 1993 Aug;122(3):729-37 PMID: 8335696
  35. Native talin is a dumbbell-shaped homodimer when it interacts with actin.
    J Struct Biol. 1994 Jan-Feb;112(1):3-10 PMID: 8031639
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.20.9161
Published
1995-09-26
Pages
9161-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC40944
Subset
IM
Grants
NCI NIH HHS · CA 42302 · United States
NCI NIH HHS · CA 54233 · United States
NCI NIH HHS · P30 CA 30199 · United States
Databases
GENBANK
L13299, L13300, L18880
External Links
PubMed source DOI: 10.1073/pnas.92.20.9161 PMC Full Text View journal details
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