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PMID: 7591150 Published · ppublish English Journal Article

Contribution of the major and minor subunits to fimbria-mediated adherence of Haemophilus influenzae to human epithelial cells and erythrocytes.

Infection and immunity ·Vol. 63 ·No. 12 ·1995-12-00 ·Pages 4883-9

van Ham SM, van Alphen L, Mooi FR, van Putten JP

Abstract

Fimbriae are colonization factors of the human pathogen Haemophilus influenzae in that they mediate bacterial adherence to human eukaryotic cells. The contribution of the major (HifA) and putative minor (HifD and HifE) subunits of H. influenzae fimbriae to fimbria-specific adherence was studied by using mutants that were inactivated in distinct fimbrial genes. Both the major and minor subunits were required for adherence of H. influenzae to oropharyngeal epithelial cells and human erythrocytes carrying the AnWj antigen. Cloning of defined H. influenzae fimbrial genes in an Escherichia coli strain with type 1 fimbriae yielded recombinants expressing high amounts of HifA-containing H. influenzae fimbriae either with or without coexpression of both H. influenzae minor subunits. Both clones exhibited the specific adherence properties of H. influenzae fimbriae, implying that the minor H. influenzae subunits are dispensable for adherence and that the adhesive domain resides in the major subunit, HifA. In H. influenzae itself, the minor subunits probably affect adherence by raising the number of fimbriae above the minimal level required to establish adherence.

MeSH Terms
Adult Bacterial Adhesion Bacterial Proteins/physiology Epithelium/microbiology Erythrocytes/microbiology Fimbriae Proteins Fimbriae, Bacterial/genetics,physiology Haemophilus influenzae/physiology Humans Multigene Family Mutation Oropharynx/cytology,microbiology
Chemicals
Bacterial Proteins HifD protein, Haemophilus influenzae HifE protein, Haemophilus influenzae Fimbriae Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
van Ham S M
Abteilung Infektionsbiologie, Max-Planck-Institut für Biologie, Tübingen, Germany.
van Alphen L
Mooi F R
van Putten J P
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.63.12.4883-4889.1995
Published
1995-12-00
Pages
4883-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC173699
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.63.12.4883-4889.1995 PMC Full Text View journal details
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