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PMID: 7625818 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mutation in the gyrA gene of quinolone-resistant clinical isolates of Acinetobacter baumannii.

Antimicrobial agents and chemotherapy ·Vol. 39 ·No. 5 ·1995-05-00 ·Pages 1201-3

Vila J, Ruiz J, Goñi P, Marcos A, Jimenez de Anta T

Abstract

The gyrA gene mutations associated with quinolone resistance were determined in 21 epidemiologically unrelated clinical isolates of Acinetobacter baumannii. Our studies highlight the conserved sequences in the quinolone resistance-determining region of the gyrA gene from A. baumannii and other bacteria. All 15 isolates for which the MIC of ciprofloxacin is > or = 4 micrograms/ml showed a change at Ser-83 to Leu. Six strains for which the MIC of ciprofloxacin is 1 microgram/ml did not show any change at Ser-83, although a strain for which the MIC of ciprofloxacin is 1 microgram/ml exhibited a change at Gly-81 to Val. Although it is possible that mutations in other locations of the gyrA gene, the gyrB gene, or in other genes may also contribute to the modulation of the MIC level, our results suggest that a gyrA mutation at Ser-83 is associated with quinolone resistance in A. baumannii.

Related Genes
gyrA
MeSH Terms
4-Quinolones Acinetobacter/drug effects,genetics Acinetobacter Infections/microbiology Amino Acid Sequence Anti-Infective Agents/pharmacology Base Sequence Humans Molecular Sequence Data Mutation/genetics Polymerase Chain Reaction
Chemicals
4-Quinolones Anti-Infective Agents
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Vila J
Departament de Microbiologia, Hospital Clinic, Facultat de Medicina, Universitat de Barcelona, Spain.
Ruiz J
Goñi P
Marcos A
Jimenez de Anta T
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.39.5.1201
Published
1995-05-00
Pages
1201-3
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC162713
Subset
IM
Databases
GENBANK
X82165
External Links
PubMed source DOI: 10.1128/AAC.39.5.1201 PMC Full Text View journal details
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