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PMID: 7630715 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Intrastrand cross-links are not formed in the reaction between transplatin and native DNA: relation with the clinical inefficiency of transplatin.

Nucleic acids research ·Vol. 23 ·No. 13 ·1995-07-11 ·Pages 2381-8

Boudvillain M, Dalbiès R, Aussourd C, Leng M

Abstract

The reaction between trans-diamminedichloroplatinum(II) and single-stranded oligonucleotides containing the sequence d(GXG) (X being an adenine, cytosine or thymine residue) yields trans-[Pt(NH3)2[(GXG)-GN7,GN7]] intrastrand cross-links. These cross-links do not prevent the pairing of the platinated oligonucleotides with their complementary strands but they decrease the thermal stability of the duplexes. The thermal stability is not much affected by the chemical nature of the X residue and its complementary base. By gel electrophoresis, it is shown that the trans- [Pt(NH3)2[d(GTG)-GN7,GN7]] cross-link bends the DNA double helix (26 degrees) and unwinds it (45 degrees). The pairing of the platinated oligonucleotides with their complementary strands promotes the rearrangement of the 1,3-intrastrand cross-links into interstrand cross-links. At a given temperature, the nature of the X residue, its complementary base and of the base pairs adjacent to the adducts do not dramatically affect the rate of the reaction. To know whether trans-[Pt(NH3)2[d(GXG)-GN7,GN7]] cross-links do not rearrange in some sequences, the location of these adducts was searched in double-stranded DNA after reaction with trans-diamminedichloroplatinum(II) by means of the 3'-5' exonuclease activity of T4 DNA polymerase. At low level of platination, trans-[Pt(NH3)2[d(GXG)-GN7,GN7]] cross-links were not detected. Monofunctional adducts and interstrand cross-links were mainly formed. These results are discussed in relation with the clinical inefficiency of trans-diamminedichloroplatinum(II).

MeSH Terms
Base Sequence Cisplatin/chemistry Cross-Linking Reagents DNA/chemistry DNA, Single-Stranded/chemistry DNA-Directed DNA Polymerase Drug Stability Hot Temperature Molecular Sequence Data Nucleic Acid Conformation Oligonucleotides/chemistry Stereoisomerism Viral Proteins/metabolism
Chemicals
Cross-Linking Reagents DNA, Single-Stranded Oligonucleotides Viral Proteins gene 43 protein, Enterobacteria phage T4 transplatin DNA DNA-Directed DNA Polymerase Cisplatin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Boudvillain M
Centre de Biophysique Moleculaire, CNRS, Orleans, France.
Dalbiès R
Aussourd C
Leng M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/23.13.2381
Published
1995-07-11
Pages
2381-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC307041
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/23.13.2381 PMC Full Text View journal details
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