Home LiteratureArticle Details
PMID: 7665485 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Involvement of cpxA, a sensor of a two-component regulatory system, in the pH-dependent regulation of expression of Shigella sonnei virF gene.

Journal of bacteriology ·Vol. 177 ·No. 17 ·1995-09-00 ·Pages 5062-9

Nakayama S, Watanabe H

Abstract

In Shigella species, IpaBCD proteins encoded on the virulence plasmid direct the entry of this bacterium into host epithelial cells. Expression of the ipaBCD genes is under the control of several environmental conditions, such as temperature and osmolarity. Extracellular pH also controlled the the expression of the genes, and this regulation occurred mainly at the step of expression of virF, a plasmid-encoded positive regulator of ipaBCD. The expression of virF was activated at high pH (pH 7.4) and repressed at low pH (pH 6.0). We isolated a Tn10 transposon mutant in Escherichia coli K-12 which altered this regulation at the transcriptional level. The Tn10 in the mutant inserted within a reading frame of the cpxA gene, whose product belongs to a family of sensor proteins of two-component signal transduction systems. Complementation analysis showed that cpxA was involved in the pH-dependent regulation of virF gene expression. A gene homologous to cpxA was conserved in Shigella spp. as well as in E. coli. These results may indicate that CpxA senses directly or indirectly a change in extracellular pH and influences the expression of virF in E. coli and Shigella spp.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/biosynthesis,metabolism Base Sequence Blotting, Southern Chromosome Mapping Escherichia coli/genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Genetic Complementation Test Hydrogen-Ion Concentration Molecular Sequence Data Mutagenesis, Insertional Protein Kinases Sequence Homology, Amino Acid Shigella sonnei/genetics,pathogenicity Signal Transduction/genetics Transcription, Genetic Virulence/genetics Virulence Factors
Chemicals
Bacterial Proteins Escherichia coli Proteins Virulence Factors Protein Kinases CpxA protein, E coli CpxA protein, bacteria
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nakayama S
Department of Bacteriology, National Institute of Health, Tokyo, Japan.
Watanabe H
References (37)
37 references, click to expand
  1. Glucose and nonmaintained pH decrease expression of the accessory gene regulator (agr) in Staphylococcus aureus.
    Infect Immun. 1992 Aug;60(8):3381-8 PMID: 1639506
  2. Genetic analysis of Escherichia coli K-12 chromosomal mutants defective in expression of F-plasmid functions: identification of genes cpxA and cpxB.
    J Bacteriol. 1980 Oct;144(1):60-7 PMID: 6998969
  3. Genetic analysis of an invasion region by use of a Tn3-lac transposon and identification of a second positive regulator gene, invE, for cell invasion of Shigella sonnei: significant homology of invE with ParB of plasmid P1.
    J Bacteriol. 1990 Feb;172(2):619-29 PMID: 1688841
  4. Salmonella typhimurium activates virulence gene transcription within acidified macrophage phagosomes.
    Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10079-83 PMID: 1438196
  5. Alkaline pH decreases expression of the accessory gene regulator (agr) in Staphylococcus aureus.
    J Bacteriol. 1992 Aug;174(15):5095-100 PMID: 1629166
  6. Nonpolar mutagenesis of the ipa genes defines IpaB, IpaC, and IpaD as effectors of Shigella flexneri entry into epithelial cells.
    J Bacteriol. 1993 Sep;175(18):5899-906 PMID: 8376337
  7. Regulation of the Escherichia coli cad operon: location of a site required for acid induction.
    J Bacteriol. 1992 Apr;174(8):2670-8 PMID: 1556086
  8. Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda.
    Genetics. 1978 Nov;90(3):427-61 PMID: 365678
  9. Large plasmids associated with virulence in Shigella species have a common function necessary for epithelial cell penetration.
    Infect Immun. 1985 Apr;48(1):260-2 PMID: 3980088
  10. The two-component regulatory system ompR-envZ controls the virulence of Shigella flexneri.
    J Bacteriol. 1990 Nov;172(11):6274-81 PMID: 2121709
  11. The deduced amino-acid sequence of the cloned cpxR gene suggests the protein is the cognate regulator for the membrane sensor, CpxA, in a two-component signal transduction system of Escherichia coli.
    Gene. 1993 Dec 22;136(1-2):227-30 PMID: 8294007
  12. Influence of pH on bacterial gene expression.
    Mol Microbiol. 1993 Apr;8(1):5-14 PMID: 8388532
  13. Conserved domains in bacterial regulatory proteins that respond to environmental stimuli.
    Cell. 1987 Jun 5;49(5):579-81 PMID: 3555840
  14. Characterization of invasion plasmid antigen genes (ipaBCD) from Shigella flexneri.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):9317-21 PMID: 3057506
  15. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
    Science. 1985 Dec 20;230(4732):1350-4 PMID: 2999980
  16. A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium.
    EMBO J. 1985 Apr;4(4):891-8 PMID: 2990912
  17. OmpC is involved in invasion of epithelial cells by Shigella flexneri.
    Infect Immun. 1993 Sep;61(9):3625-35 PMID: 8359885
  18. Transcriptional control of the invasion regulatory gene virB of Shigella flexneri: activation by virF and repression by H-NS.
    J Bacteriol. 1993 Oct;175(19):6142-9 PMID: 7691791
  19. The cpx proteins of Escherichia coli K12. Structure of the cpxA polypeptide as an inner membrane component.
    J Mol Biol. 1988 Sep 20;203(2):467-78 PMID: 3058985
  20. The Cpx two-component signal transduction pathway of Escherichia coli regulates transcription of the gene specifying the stress-inducible periplasmic protease, DegP.
    Genes Dev. 1995 Feb 15;9(4):387-98 PMID: 7883164
  21. Identification of elements involved in transcriptional regulation of the Escherichia coli cad operon by external pH.
    J Bacteriol. 1992 Jan;174(2):530-40 PMID: 1370290
  22. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  23. Environmental signals controlling expression of virulence determinants in bacteria.
    J Bacteriol. 1992 Jan;174(1):1-7 PMID: 1729202
  24. A role for H-NS in the thermo-osmotic regulation of virulence gene expression in Shigella flexneri.
    J Bacteriol. 1994 Jul;176(13):4187-91 PMID: 8021202
  25. Prokaryotic signal transduction mediated by sensor and regulator protein pairs.
    Annu Rev Genet. 1989;23:311-36 PMID: 2694934
  26. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.
    Cell. 1987 Jul 31;50(3):495-508 PMID: 3038334
  27. The Cpx proteins of Escherichia coli K-12: evidence that cpxA, ecfB, ssd, and eup mutations all identify the same gene.
    J Bacteriol. 1990 May;172(5):2456-61 PMID: 2185221
  28. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  29. Organization of structural and regulatory genes that mediate tetracycline resistance in transposon Tn10.
    J Bacteriol. 1979 Jun;138(3):705-14 PMID: 378932
  30. EPITHELIAL CELL PENETRATION AS AN ESSENTIAL STEP IN THE PATHOGENESIS OF BACILLARY DYSENTERY.
    J Bacteriol. 1964 Nov;88(5):1503-18 PMID: 16562000
  31. A dual transcriptional activation system for the 230 kb plasmid genes coding for virulence-associated antigens of Shigella flexneri.
    Mol Microbiol. 1989 May;3(5):627-35 PMID: 2474742
  32. Genetic regulation of bacterial virulence.
    Annu Rev Genet. 1989;23:455-82 PMID: 2694940
  33. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  34. Cloning of regions required for contact hemolysis and entry into LLC-MK2 cells from Shigella sonnei form I plasmid: virF is a positive regulator gene for these phenotypes.
    Infect Immun. 1989 May;57(5):1391-8 PMID: 2651305
  35. Protein phosphorylation and regulation of adaptive responses in bacteria.
    Microbiol Rev. 1989 Dec;53(4):450-90 PMID: 2556636
  36. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  37. DNA sequence organization of IS10-right of Tn10 and comparison with IS10-left.
    Proc Natl Acad Sci U S A. 1982 Apr;79(8):2608-12 PMID: 6283536
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.17.5062-5069.1995
Published
1995-09-00
Pages
5062-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177285
Subset
IM
Databases
GENBANK
S79443
External Links
PubMed source DOI: 10.1128/jb.177.17.5062-5069.1995 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2