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PMID: 7682214 Published · ppublish English Journal Article

Tyrosine phosphate in a- and b-type flagellins of Pseudomonas aeruginosa.

Journal of bacteriology ·Vol. 175 ·No. 8 ·1993-04-00 ·Pages 2458-61

Kelly-Wintenberg K, South SL, Montie TC

Abstract

Both a- and b-type purified flagellins from a number of Pseudomonas aeruginosa strains grown in radiolabeled phosphate were shown to be phosphorylated. Analysis of partial acid-hydrolyzed flagellar filaments revealed that 32Pi was in phosphotyrosine. Three 32P-phosphopeptides apparently are common to a- and b-type flagellins, but a fourth peptide was found only in b-type hydrolysates. P. aeruginosa PAK flagellin, containing only two tyrosines, both in the variable region, was readily labeled and gave the same peptide pattern as flagellins containing additional tyrosines. Data showing that a- and b-type flagellins gave positive immunoblots with antiphosphotyrosine monoclonal antibody and that release of P(i) by alkaline phosphatase occurred indicated that unmodified tyrosine phosphate exists in flagellin.

MeSH Terms
Antibodies, Monoclonal/immunology Flagella/metabolism Flagellin/chemistry,isolation & purification Immunoblotting Phosphoric Monoester Hydrolases/metabolism Phosphorylation Phosphotyrosine Pseudomonas aeruginosa/chemistry,metabolism Trypsin/pharmacology Tyrosine/analogs & derivatives,analysis
Chemicals
Antibodies, Monoclonal Flagellin Phosphotyrosine Tyrosine Phosphoric Monoester Hydrolases Trypsin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kelly-Wintenberg K
Department of Microbiology, University of Tennessee, Knoxville 37996.
South S L
Montie T C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.8.2458-2461.1993
Published
1993-04-00
Pages
2458-61
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC204538
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.175.8.2458-2461.1993 PMC Full Text View journal details
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