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PMID: 7684459 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Epstein-Barr virus latent membrane protein 2A blocks calcium mobilization in B lymphocytes.

Journal of virology ·Vol. 67 ·No. 6 ·1993-06-00 ·Pages 3087-94

Miller CL, Longnecker R, Kieff E

Abstract

LMP2A is expressed in latent Epstein-Barr virus (EBV) infection and interacts with LMP1 and members of the src tyrosine kinase family in the plasma membrane. Since tyrosine kinase mediate receptor-induced changes in intracellular free calcium, the effect of LMP2A on receptor-mediated intracellular calcium mobilization was evaluated by stably expressing LMP2A in an EBV-negative Burkitt tumor cell line (BJAB) or in LMP1-converted BJAB cells. LMP2A significantly blocked calcium mobilization following class II, CD19, or immunoglobulin M cross-linking. LMP2A effects were partially reversed in LMP1-converted cell lines. These results are compatible with LMP2A acting in latent B-lymphocyte infection to downmodulate LMP1 effects on cell growth or to inhibit induction of lytic EBV infection in specific human tissues following receptor ligation.

MeSH Terms
Antigens, CD/metabolism Antigens, CD19 Antigens, Differentiation, B-Lymphocyte/metabolism Antigens, Viral/genetics,metabolism,pharmacology B-Lymphocytes/drug effects,metabolism Burkitt Lymphoma Calcium/metabolism Down-Regulation Flow Cytometry Genes, MHC Class II Humans Immunoglobulin M/metabolism Lymphocyte Activation/physiology Receptors, Cell Surface/metabolism Transfection Tumor Cells, Cultured Viral Matrix Proteins/genetics,metabolism,pharmacology
Chemicals
Antigens, CD Antigens, CD19 Antigens, Differentiation, B-Lymphocyte Antigens, Viral EBV-associated membrane antigen, Epstein-Barr virus Immunoglobulin M Receptors, Cell Surface Viral Matrix Proteins Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Miller C L
Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115.
Longnecker R
Kieff E
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.6.3087-3094.1993
Published
1993-06-00
Pages
3087-94
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC237645
Subset
IM
Grants
NCI NIH HHS · CA 47006 · United States
External Links
PubMed source DOI: 10.1128/JVI.67.6.3087-3094.1993 PMC Full Text View journal details
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