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PMID: 1321296 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An Epstein-Barr virus transformation-associated membrane protein interacts with src family tyrosine kinases.

Journal of virology ·Vol. 66 ·No. 8 ·1992-08-00 ·Pages 5161-7

Burkhardt AL, Bolen JB, Kieff E, Longnecker R

Abstract

In latently infected growth-transformed human lymphocytes, Epstein-Barr virus (EBV) encodes two integral plasma membrane proteins: LMP1, which constitutively induces B-lymphocyte activation and intercellular adhesion, and LMP2A, which associates with LMP1 and is a tyrosine kinase substrate. We now demonstrate that LMP2A associates with src family protein tyrosine kinases, particularly lyn kinase, in nonionic detergent extracts of transfected B lymphoma cells or in extracts of EBV-transformed B lymphocytes. The LMP2A and tyrosine kinase association is stable in nonionic detergents and includes a 70-kDa cell protein which is also an in vitro or in vivo kinase substrate. This LMP2A association with B-lymphocyte src family tyrosine kinases is likely to be an important pathway in EBV's effects on cell growth.

Related Genes
src
MeSH Terms
Animals Antigens, Viral/genetics,isolation & purification,metabolism B-Lymphocytes Cell Line Cell Transformation, Viral Genes, src Herpesvirus 4, Human/metabolism Lymphoma, B-Cell Membrane Proteins/genetics,isolation & purification,metabolism Oncogene Protein pp60(v-src)/isolation & purification,metabolism Protein Binding Protein-Tyrosine Kinases/isolation & purification,metabolism Transfection Viral Matrix Proteins Viral Proteins/genetics,isolation & purification,metabolism
Chemicals
Antigens, Viral EBV-associated membrane antigen, Epstein-Barr virus Membrane Proteins Viral Matrix Proteins Viral Proteins Protein-Tyrosine Kinases Oncogene Protein pp60(v-src)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Burkhardt A L
Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543.
Bolen J B
Kieff E
Longnecker R
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.8.5161-5167.1992
Published
1992-08-00
Pages
5161-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC241398
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.66.8.5161-5167.1992 PMC Full Text View journal details
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