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PMID: 7685337 Published · ppublish English Comparative Study Journal Article

Transcriptional analysis of the gap-pgk-tpi-ppc gene cluster of Corynebacterium glutamicum.

Journal of bacteriology ·Vol. 175 ·No. 12 ·1993-06-00 ·Pages 3905-8

Schwinde JW, Thum-Schmitz N, Eikmanns BJ, Sahm H

Abstract

The transcriptional organization of the Corynebacterium glutamicum gap-pgk-tpi-ppc gene cluster, encoding glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate kinase, triosephosphate isomerase, and phosphoenolpyruvate carboxylase, was investigated by Northern (RNA) blot and primer extension analyses. Four transcripts corresponding to gap, to gap-pgk-tpi, to pgk-tpi, and to pgk-tpi-ppc were identified. The respective transcriptional initiation sites in front of gap and pgk were located, and, from the analysis of DNA sequences upstream of these and of previously determined transcriptional start sites, common structures which may be important for promoter function in C. glutamicum are discussed.

Related Genes
gap pgk ppc tpi
MeSH Terms
Bacillus subtilis/genetics Base Sequence Consensus Sequence Corynebacterium/genetics DNA, Bacterial/genetics Escherichia coli/genetics Gene Expression Regulation, Bacterial Genes, Bacterial Glycolysis Molecular Sequence Data Multigene Family Promoter Regions, Genetic RNA, Bacterial/genetics RNA, Messenger/genetics Restriction Mapping Sequence Alignment Transcription, Genetic
Chemicals
DNA, Bacterial RNA, Bacterial RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schwinde J W
Institut für Biotechnologie 1 des Forschungszentrums Jülich GmbH, Germany.
Thum-Schmitz N
Eikmanns B J
Sahm H
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.12.3905-3908.1993
Published
1993-06-00
Pages
3905-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC204809
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.175.12.3905-3908.1993 PMC Full Text View journal details
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