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PMID: 7707498 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The p6gag domain of human immunodeficiency virus type 1 is sufficient for the incorporation of Vpr into heterologous viral particles.

Journal of virology ·Vol. 69 ·No. 5 ·1995-05-00 ·Pages 2759-64

Kondo E, Mammano F, Cohen EA, Göttlinger HG

Abstract

The vpr gene of human immunodeficiency virus type 1 (HIV-1) encodes a virion-associated regulatory protein. Mutagenesis has shown that the virion association of Vpr requires sequences near the C terminus of the HIV-1 Gag polyprotein Pr55gag. To investigate whether Vpr incorporation is mediated by a specific domain of Pr55gag, we examined the ability of chimeric HIV-1/Moloney murine leukemia virus (MLV) Gag polyproteins to direct the incorporation of Vpr. Vpr expressed in trans did not associate with particles formed by the authentic MLV Gag polyprotein or with particles formed by chimeric Gag polyproteins that had the matrix (MA) or capsid (CA) domain of MLV precisely replaced by the corresponding domain of HIV-1HXB2. By contrast, Vpr was efficiently incorporated upon replacement of the C-terminal nucleocapsid (NC) domain of the MLV Gag polyprotein with HIV-1 p15 sequences. Vpr was also efficiently incorporated into particles formed by a MLV Gag polyprotein that had the HIV-1 p6 domain fused to its C terminus. Furthermore, a deletion analysis revealed that a conserved region near the C terminus of the p6 domain is essential for Vpr incorporation, whereas sequences downstream of the conserved region are dispensable. These results show that a virion association motif for Vpr is located within residues 1 to 46 of p6.

MeSH Terms
Amino Acid Sequence Animals Cell Line Conserved Sequence Gene Products, gag/genetics,physiology Gene Products, vpr/genetics,physiology Genes, gag Genes, vpr HIV-1/genetics,physiology Molecular Sequence Data Recombinant Fusion Proteins/genetics,physiology Virus Replication/genetics,physiology gag Gene Products, Human Immunodeficiency Virus vpr Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, gag Gene Products, vpr Recombinant Fusion Proteins gag Gene Products, Human Immunodeficiency Virus p6 gag protein, Human immunodeficiency virus 1 vpr Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kondo E
Division of Human Retrovirology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
Mammano F
Cohen E A
Göttlinger H G
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.69.5.2759-2764.1995
Published
1995-05-00
Pages
2759-64
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC188969
Subset
IM
Grants
NIAID NIH HHS · AI28691 · United States
NIAID NIH HHS · AI29873 · United States
NIAID NIH HHS · AI34267 · United States
External Links
PubMed source DOI: 10.1128/JVI.69.5.2759-2764.1995 PMC Full Text View journal details
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