Home LiteratureArticle Details
PMID: 8411353 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Incorporation of human immunodeficiency virus type 1 Gag proteins into murine leukemia virus virions.

Journal of virology ·Vol. 67 ·No. 11 ·1993-11-00 ·Pages 6499-506

Deminie CA, Emerman M

Abstract

The retroviral Gag polyprotein is necessary and sufficient for assembly and budding of viral particles. However, the exact inter- and intramolecular interactions of the Gag polyproteins during this process are not known. To locate functional domains within Gag, we generated chimeric proviruses between human immunodeficiency virus type 1 (HIV-1) and murine leukemia virus (MuLV). In these chimeric proviruses, the matrix or capsid proteins of MuLV were precisely replaced with the matrix or capsid proteins of HIV-1. Although the chimeric proviruses were unable to efficiently assemble into mature viral particles by themselves, coexpression of wild-type MuLV Gag rescued the HIV proteins into virions. The specificity of the rescue of HIV proteins into MuLV virions shows that specific interactions involving homologous matrix or capsid regions of Gag are necessary for retroviral particle formation.

Related Genes
gag
MeSH Terms
3T3 Cells Animals Base Sequence Capsid/metabolism Gene Products, gag/metabolism HIV-1/growth & development,metabolism Helper Viruses/metabolism Leukemia Virus, Murine/growth & development,metabolism Mice Molecular Sequence Data Oligodeoxyribonucleotides/chemistry RNA, Viral/metabolism Recombinant Fusion Proteins/metabolism Transfection Viral Matrix Proteins/metabolism Virus Replication
Chemicals
Gene Products, gag Oligodeoxyribonucleotides RNA, Viral Recombinant Fusion Proteins Viral Matrix Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Deminie C A
Program in Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104-2092.
Emerman M
References (35)
35 references, click to expand
  1. Human immunodeficiency virus type 1 Pr55gag and Pr160gag-pol expressed from a simian virus 40 late replacement vector are efficiently processed and assembled into viruslike particles.
    J Virol. 1990 Jun;64(6):2743-50 PMID: 1692347
  2. Assembly-defective point mutants of the human immunodeficiency virus type 1 Gag precursor phenotypically expressed in recombinant baculovirus-infected cells.
    J Virol. 1993 May;67(5):2787-98 PMID: 8474175
  3. Structural role of the matrix protein of type D retroviruses in gag polyprotein stability and capsid assembly.
    J Virol. 1990 Sep;64(9):4383-9 PMID: 2200887
  4. Transport and assembly of gag proteins into Moloney murine leukemia virus.
    J Virol. 1990 Nov;64(11):5306-16 PMID: 1698996
  5. Amino acid substitutions within the matrix protein of type D retroviruses affect assembly, transport and membrane association of a capsid.
    EMBO J. 1991 Mar;10(3):535-46 PMID: 1705884
  6. Effect of mutations affecting the p6 gag protein on human immunodeficiency virus particle release.
    Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3195-9 PMID: 2014240
  7. Expression of active human immunodeficiency virus type 1 protease by noninfectious chimeric virus particles.
    J Virol. 1991 Jun;65(6):3007-14 PMID: 1709693
  8. Suppression of retroviral MA deletions by the amino-terminal membrane-binding domain of p60src.
    J Virol. 1991 Jul;65(7):3804-12 PMID: 1710290
  9. Assembly and morphology of HIV: potential effect of structure on viral function.
    AIDS. 1991 Jun;5(6):617-37 PMID: 1652977
  10. Form, function, and use of retroviral gag proteins.
    AIDS. 1991 Jun;5(6):639-54 PMID: 1883539
  11. Identification and characterization of intragenic sequences which repress human immunodeficiency virus structural gene expression.
    J Virol. 1991 Oct;65(10):5305-13 PMID: 1895385
  12. Localization of RNA tumor virus polypeptides. I. Isolation of further virus substructures.
    Virology. 1973 Dec;56(2):549-64 PMID: 4127970
  13. Isolation and properties of Moloney murine leukemia virus mutants: use of a rapid assay for release of virion reverse transcriptase.
    J Virol. 1981 Apr;38(1):239-48 PMID: 6165830
  14. Mutations in gag proteins P12 and P15 of Moloney murine leukemia virus block early stages of infection.
    J Virol. 1984 Mar;49(3):909-17 PMID: 6608006
  15. Mutations in the gag gene of Moloney murine leukemia virus: effects on production of virions and reverse transcriptase.
    J Virol. 1984 Mar;49(3):918-24 PMID: 6199513
  16. Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS.
    Science. 1984 May 4;224(4648):497-500 PMID: 6200935
  17. Point mutations in the P30 domain of the gag gene of Moloney murine leukemia virus.
    Virology. 1985 Apr 15;142(1):211-4 PMID: 2414902
  18. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  19. High-efficiency transformation of mammalian cells by plasmid DNA.
    Mol Cell Biol. 1987 Aug;7(8):2745-52 PMID: 3670292
  20. Standardized and simplified nomenclature for proteins common to all retroviruses.
    J Virol. 1988 May;62(5):1808-9 PMID: 3357211
  21. Unmyristylated Moloney murine leukemia virus Pr65gag is excluded from virus assembly and maturation events.
    J Virol. 1989 May;63(5):2370-3 PMID: 2649693
  22. A nucleoprotein complex mediates the integration of retroviral DNA.
    Genes Dev. 1989 Apr;3(4):469-78 PMID: 2721960
  23. HIV-1 Gag mutants can dominantly interfere with the replication of the wild-type virus.
    Cell. 1989 Oct 6;59(1):113-20 PMID: 2676192
  24. Myristoylation-dependent replication and assembly of human immunodeficiency virus 1.
    Proc Natl Acad Sci U S A. 1990 Jan;87(2):523-7 PMID: 2405382
  25. Assembly of gag-beta-galactosidase proteins into retrovirus particles.
    J Virol. 1990 May;64(5):2265-79 PMID: 2109101
  26. Identification of posttranscriptionally active inhibitory sequences in human immunodeficiency virus type 1 RNA: novel level of gene regulation.
    J Virol. 1991 Nov;65(11):5732-43 PMID: 1656066
  27. Changes in growth properties on passage in tissue culture of viruses derived from infectious molecular clones of HIV-1LAI, HIV-1MAL, and HIV-1ELI.
    Virology. 1991 Dec;185(2):661-72 PMID: 1683726
  28. Distinct RNA sequences in the gag region of human immunodeficiency virus type 1 decrease RNA stability and inhibit expression in the absence of Rev protein.
    J Virol. 1992 Jan;66(1):150-9 PMID: 1727477
  29. Matrix protein of Akv murine leukemia virus: genetic mapping of regions essential for particle formation.
    J Virol. 1992 Jul;66(7):4479-87 PMID: 1318415
  30. The matrix protein of human immunodeficiency virus type 1 is required for incorporation of viral envelope protein into mature virions.
    J Virol. 1992 Aug;66(8):4966-71 PMID: 1629961
  31. Human immunodeficiency virus infection of cells arrested in the cell cycle.
    EMBO J. 1992 Aug;11(8):3053-8 PMID: 1322294
  32. Mutational analysis of the major homology region of Mason-Pfizer monkey virus by use of saturation mutagenesis.
    J Virol. 1992 Dec;66(12):7021-32 PMID: 1279197
  33. A subset of Pr65gag is nucleus associated in murine leukemia virus-infected cells.
    J Virol. 1993 Mar;67(3):1350-6 PMID: 8437220
  34. Identification of a region in the Pr55gag-polyprotein essential for HIV-1 particle formation.
    Virology. 1993 Apr;193(2):981-5 PMID: 8460500
  35. Autoradiography using storage phosphor technology.
    Electrophoresis. 1990 May;11(5):355-60 PMID: 2194789
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.11.6499-6506.1993
Published
1993-11-00
Pages
6499-506
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC238086
Subset
IM
Grants
NIAID NIH HHS · AI08587 · United States
NIAID NIH HHS · R01 AI30927 · United States
External Links
PubMed source DOI: 10.1128/JVI.67.11.6499-6506.1993 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2