Home LiteratureArticle Details
PMID: 1322294 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human immunodeficiency virus infection of cells arrested in the cell cycle.

The EMBO journal ·Vol. 11 ·No. 8 ·1992-08-00 ·Pages 3053-8

Lewis P, Hensel M, Emerman M

Abstract

Cell proliferation is necessary for proviral integration and productive infection of most retroviruses. Nevertheless, the human immunodeficiency virus (HIV) can infect non-dividing macrophages. This ability to grow in non-dividing cells is not specific to macrophages because, as we show here, CD4+ HeLa cells arrested at stage G2 of the cell cycle can be infected by HIV-1. Proliferation is necessary for these same cells to be infected by a murine retrovirus, MuLV. HIV-1 integrates into the arrested cell DNA and produces viral RNA and protein in a pattern similar to that in normal cells. In addition, our data suggest that the ability to infect non-dividing cells is due to one of the HIV-1 core virion proteins. HIV infection of non-dividing cells distinguishes lentiviruses from other retroviruses and is likely to be important in the natural history of HIV infection.

Related Genes
tat
MeSH Terms
Base Sequence CD4 Antigens/physiology Cell Cycle/physiology,radiation effects Cell Division/physiology Chromosome Deletion G2 Phase Genes, Viral Genes, tat HIV-1/genetics,physiology HeLa Cells Humans Leukemia Virus, Murine/genetics,physiology Molecular Sequence Data Oligodeoxyribonucleotides Plasmids Polymerase Chain Reaction/methods Virus Replication/radiation effects beta-Galactosidase/biosynthesis,genetics
Chemicals
CD4 Antigens Oligodeoxyribonucleotides beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lewis P
Department of Pediatrics, University of Washington, Seattle.
Hensel M
Emerman M
References (40)
40 references, click to expand
  1. Functional plasticity of microglia: a review.
    Glia. 1988;1(5):301-7 PMID: 2976393
  2. Replication of human immunodeficiency virus type 1 in primary dendritic cell cultures.
    Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7998-8002 PMID: 1910172
  3. Detection of AIDS virus in macrophages in brain tissue from AIDS patients with encephalopathy.
    Science. 1986 Sep 5;233(4768):1089-93 PMID: 3016903
  4. The brain in AIDS: central nervous system HIV-1 infection and AIDS dementia complex.
    Science. 1988 Feb 5;239(4840):586-92 PMID: 3277272
  5. Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production.
    Mol Cell Biol. 1986 Aug;6(8):2895-902 PMID: 3785217
  6. Cellular tropism of the human retrovirus HTLV-III/LAV. I. Role of T cell activation and expression of the T4 antigen.
    J Immunol. 1985 Nov;135(5):3151-62 PMID: 2995487
  7. Cellular functions are required for the synthesis and integration of avian sarcoma virus-specific DNA.
    Cell. 1977 Jun;11(2):307-19 PMID: 196759
  8. Changes in growth properties on passage in tissue culture of viruses derived from infectious molecular clones of HIV-1LAI, HIV-1MAL, and HIV-1ELI.
    Virology. 1991 Dec;185(2):661-72 PMID: 1683726
  9. In vitro maturation of mononuclear phagocytes and susceptibility to HIV-1 infection.
    J Acquir Immune Defic Syndr. 1991;4(8):751-9 PMID: 1856787
  10. Determination of viral proteins present in the human immunodeficiency virus type 1 preintegration complex.
    J Virol. 1991 Apr;65(4):1910-5 PMID: 2002549
  11. Activation of a beta-galactosidase recombinant provirus: application to titration of human immunodeficiency virus (HIV) and HIV-infected cells.
    J Virol. 1990 Jun;64(6):2660-8 PMID: 2110596
  12. HIV-1 replication is controlled at the level of T cell activation and proviral integration.
    EMBO J. 1990 May;9(5):1551-60 PMID: 2184033
  13. HIV-1 entry into quiescent primary lymphocytes: molecular analysis reveals a labile, latent viral structure.
    Cell. 1990 Apr 20;61(2):213-22 PMID: 2331748
  14. Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection.
    Mol Cell Biol. 1990 Aug;10(8):4239-42 PMID: 2370865
  15. Infection of monocyte/macrophages by human T lymphotropic virus type III.
    J Clin Invest. 1986 May;77(5):1712-5 PMID: 2422213
  16. Improved retroviral vectors for gene transfer and expression.
    Biotechniques. 1989 Oct;7(9):980-2, 984-6, 989-90 PMID: 2631796
  17. A nucleoprotein complex mediates the integration of retroviral DNA.
    Genes Dev. 1989 Apr;3(4):469-78 PMID: 2721960
  18. The rev gene product of the human immunodeficiency virus affects envelope-specific RNA localization.
    Cell. 1989 Jun 30;57(7):1155-65 PMID: 2736624
  19. The relation of protein synthesis to radiation-induced division delay in Chinese hamster cells.
    Radiat Res. 1970 Dec;44(3):788-806 PMID: 5530793
  20. Slow virus visna: reproduction in vitro of virus from extrachromosomal DNA.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7212-5 PMID: 6095275
  21. Visna virus synthesized in absence of host-cell division and DNA synthesis.
    Microbios. 1980;29(116):71-80 PMID: 6267436
  22. Establishment of infection by spleen necrosis virus: inhibition in stationary cells and the role of secondary infection.
    J Virol. 1982 Jan;41(1):183-91 PMID: 6283112
  23. An X-linked gene affecting mouse cell DNA synthesis also affects production of unintegrated linear and supercoiled DNA of murine leukemia virus.
    Mol Cell Biol. 1984 Jan;4(1):151-9 PMID: 6538258
  24. Alveolar macrophage replication. One mechanism for the expansion of the mononuclear phagocyte population in the chronically inflamed lung.
    J Clin Invest. 1984 Aug;74(2):460-9 PMID: 6746904
  25. Detection of replication-competent and pseudotyped human immunodeficiency virus with a sensitive cell line on the basis of activation of an integrated beta-galactosidase gene.
    J Virol. 1992 Apr;66(4):2232-9 PMID: 1548759
  26. Productive human immunodeficiency virus type 1 (HIV-1) infection of nonproliferating human monocytes.
    J Exp Med. 1991 Dec 1;174(6):1477-82 PMID: 1720811
  27. Integration is not necessary for expression of human immunodeficiency virus type 1 protein products.
    J Virol. 1990 May;64(5):2421-5 PMID: 2157898
  28. Specific tropism of HIV-1 for microglial cells in primary human brain cultures.
    Science. 1990 Aug 3;249(4968):549-53 PMID: 2200125
  29. 3'-Azido-3'-deoxythymidine (BW A509U): an antiviral agent that inhibits the infectivity and cytopathic effect of human T-lymphotropic virus type III/lymphadenopathy-associated virus in vitro.
    Proc Natl Acad Sci U S A. 1985 Oct;82(20):7096-100 PMID: 2413459
  30. New retrovirus helper cells with almost no nucleotide sequence homology to retrovirus vectors.
    J Virol. 1989 Jul;63(7):3209-12 PMID: 2524600
  31. The reservoir for HIV-1 in human peripheral blood is a T cell that maintains expression of CD4.
    Science. 1989 Jul 21;245(4915):305-8 PMID: 2665081
  32. Genetic recombination of human immunodeficiency virus.
    J Virol. 1989 Mar;63(3):1455-9 PMID: 2915387
  33. Nucleotide sequence of the AIDS virus, LAV.
    Cell. 1985 Jan;40(1):9-17 PMID: 2981635
  34. The role of mononuclear phagocytes in HTLV-III/LAV infection.
    Science. 1986 Jul 11;233(4760):215-9 PMID: 3014648
  35. AIDS subacute encephalitis. Identification of HIV-infected cells.
    Am J Pathol. 1987 Mar;126(3):403-10 PMID: 3548405
  36. Light inactivation of focus formation by chicken embryo fibroblasts infected with avian sarcoma virus in the presence of 5-bromodeoxyuridine.
    Nature. 1970 Nov 14;228(5272):622-4 PMID: 4319842
  37. A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis.
    Cytometry. 1983 Mar;3(5):323-7 PMID: 6188586
  38. Visna DNA synthesis and the tempo of infection in vitro.
    Virology. 1982 Jun;119(2):399-410 PMID: 6281982
  39. Effect of aphidicolin on avian sarcoma virus replication.
    J Virol. 1982 Nov;44(2):493-8 PMID: 6292514
  40. A radiological study of cell-virus interaction in the Rous sarcoma.
    Virology. 1959 Jan;7(1):75-91 PMID: 13636057
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-08-00
Pages
3053-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556788
Subset
IM
Grants
NICHD NIH HHS · HD07233 · United States
NIAID NIH HHS · P01 AI27291 · United States
NIAID NIH HHS · R01 AI30927 · United States
Corrections
ErratumIn
-
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2