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PMID: 2157898 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Integration is not necessary for expression of human immunodeficiency virus type 1 protein products.

Journal of virology ·Vol. 64 ·No. 5 ·1990-05-00 ·Pages 2421-5

Stevenson M, Haggerty S, Lamonica CA, Meier CM, Welch SK, Wasiak AJ

Abstract

A common feature in the life cycle of cytocidal retroviruses, including human immunodeficiency virus type 1 (HIV-1), is the accumulation of large amounts of unintegrated viral DNA. As yet, the role of unintegrated viral DNA in the cytopathogenesis of cytocidal retrovirus infections remains unresolved. HIV-1 mutants which were deleted in the integrase/endonuclease gene and which were unable to establish an integrated form of the virus were constructed. Despite an inability to integrate, these mutants were fully competent templates for HIV-1 core and envelope antigen production. HIV-1 antigen could be detected in the supernatants of lymphocyte cultures infected with HIV-1 integrase mutants. However, an inability to rescue infectious virus from these cultures indicated that HIV-1 integration was required for the production of infectious HIV-1. On the basis of the ability of unintegrated HIV-1 DNA to serve as a template for HIV-1 antigen production, it is plausible that unintegrated viral DNA can contribute to the HIV-1 antigen pool during HIV-1 replication.

MeSH Terms
CD4 Antigens/analysis Cell Line Chromosome Deletion DNA Transposable Elements DNA, Viral/genetics Gene Expression Genes, Viral HIV-1/genetics,physiology HeLa Cells/metabolism Humans Lysogeny Mutation Polymerase Chain Reaction Transfection Viral Proteins/genetics Viral Structural Proteins/genetics
Chemicals
CD4 Antigens DNA Transposable Elements DNA, Viral Viral Proteins Viral Structural Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Stevenson M
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68105-1065.
Haggerty S
Lamonica C A
Meier C M
Welch S K
Wasiak A J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.5.2421-2425.1990
Published
1990-05-00
Pages
2421-5
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249408
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.64.5.2421-2425.1990 PMC Full Text View journal details
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