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PMID: 2145446 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Open reading frame vpr of simian immunodeficiency virus encodes a virion-associated protein.

Journal of virology ·Vol. 64 ·No. 11 ·1990-11-00 ·Pages 5688-93

Yu XF, Matsuda M, Essex M, Lee TH

Abstract

The genomes of simian immunodeficiency viruses isolated from rhesus macaques (SIVmac) contain an open reading frame (ORF), vpr, which has a coding potential of 97 to 101 amino acid residues. In this study, a vpr ORF-encoded protein of approximately 11 kDa was identified, and anti-vpr antibodies were detected in rhesus macaques infected by SIVmac. These results provide clear evidence that the vpr ORF is a coding gene of SIVmac. The vpr protein, like the vpx protein which is encoded by another accessory gene of SIVmac, was also found to be associated with viral particles. This observation demonstrates that more than one accessory gene product can be present in the virions of this family of retroviruses and raises the possibility that the vpr protein may have a role in early part of the virus life cycle.

MeSH Terms
Base Sequence Blotting, Western Cloning, Molecular Gene Products, vpr/analysis,genetics Genes, Regulator Genes, Viral Genes, vpr Genetic Vectors Molecular Sequence Data Oligonucleotides Simian Immunodeficiency Virus/genetics Viral Structural Proteins/genetics Virus Replication
Chemicals
Gene Products, vpr Oligonucleotides Viral Structural Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yu X F
Department of Cancer Biology, Harvard School of Public Health, Boston, Massachusetts 02115.
Matsuda M
Essex M
Lee T H
References (42)
42 references, click to expand
  1. Viral protein R of human immunodeficiency virus types 1 and 2 is dispensable for replication and cytopathogenicity in lymphoid cells.
    J Virol. 1989 Jul;63(7):3205-8 PMID: 2524599
  2. The human immunodeficiency virus type 2 (HIV-2) contains a novel gene encoding a 16 kD protein associated with mature virions.
    AIDS Res Hum Retroviruses. 1988 Aug;4(4):243-50 PMID: 3061415
  3. Structure of simian immunodeficiency virus regulatory genes.
    Proc Natl Acad Sci U S A. 1989 Jul;86(13):4813-7 PMID: 2544874
  4. Mutational analysis of the human immunodeficiency virus vpr open reading frame.
    J Virol. 1989 Sep;63(9):4110-4 PMID: 2474678
  5. Functional comparison of the Rev trans-activators encoded by different primate immunodeficiency virus species.
    Proc Natl Acad Sci U S A. 1989 Nov;86(21):8222-6 PMID: 2682638
  6. Identification of HIV-1 vpr product and function.
    J Acquir Immune Defic Syndr. 1990;3(1):11-8 PMID: 2136707
  7. Mutational analysis of the human immunodeficiency virus type 2 (HIV-2) genome in relation to HIV-1 and simian immunodeficiency virus SIV (AGM).
    J Virol. 1990 Feb;64(2):742-7 PMID: 2296082
  8. CD4-independent, productive infection of a neuronal cell line by human immunodeficiency virus type 1.
    J Virol. 1990 Mar;64(3):1383-7 PMID: 2304148
  9. A tripartite HIV-1 tat-env-rev fusion protein.
    EMBO J. 1990 Mar;9(3):965-70 PMID: 2178928
  10. A novel human immunodeficiency virus type 1 protein, tev, shares sequences with tat, env, and rev proteins.
    J Virol. 1990 Jun;64(6):2505-18 PMID: 2186172
  11. Human immunodeficiency virus vpr product is a virion-associated regulatory protein.
    J Virol. 1990 Jun;64(6):3097-9 PMID: 2139896
  12. The N-terminal region of the human immunodeficiency virus envelope glycoprotein gp120 contains potential binding sites for CD4.
    Proc Natl Acad Sci U S A. 1990 May;87(10):3695-9 PMID: 2339113
  13. Human T-cell leukemia virus-associated membrane antigens: identity of the major antigens recognized after virus infection.
    Proc Natl Acad Sci U S A. 1984 Jun;81(12):3856-60 PMID: 6328528
  14. Transmission of HTLV-III infection from human plasma to chimpanzees: an animal model for AIDS.
    Science. 1984 Nov 2;226(4674):549-52 PMID: 6093251
  15. Infection of chimpanzees by human T-lymphotropic retroviruses in brain and other tissues from AIDS patients.
    Lancet. 1985 Jan 5;1(8419):55-6 PMID: 2856985
  16. Antibodies to simian T-lymphotropic retrovirus type III in African green monkeys and recognition of STLV-III viral proteins by AIDS and related sera.
    Lancet. 1985 Jun 8;1(8441):1330-2 PMID: 2860515
  17. Trans-activator gene of human T-lymphotropic virus type III (HTLV-III).
    Science. 1985 Jul 5;229(4708):69-73 PMID: 2990040
  18. Location of the trans-activating region on the genome of human T-cell lymphotropic virus type III.
    Science. 1985 Jul 5;229(4708):74-7 PMID: 2990041
  19. A new HTLV-III/LAV encoded antigen detected by antibodies from AIDS patients.
    Science. 1985 Nov 15;230(4727):810-3 PMID: 2997921
  20. A new HTLV-III/LAV protein encoded by a gene found in cytopathic retroviruses.
    Science. 1986 Mar 28;231(4745):1546-9 PMID: 3006243
  21. Replicative and cytopathic potential of HTLV-III/LAV with sor gene deletions.
    Science. 1986 Mar 28;231(4745):1549-53 PMID: 3006244
  22. Identification of HTLV-III/LAV sor gene product and detection of antibodies in human sera.
    Science. 1986 Mar 28;231(4745):1553-5 PMID: 3006245
  23. Infection of monocyte/macrophages by human T lymphotropic virus type III.
    J Clin Invest. 1986 May;77(5):1712-5 PMID: 2422213
  24. A second post-transcriptional trans-activator gene required for HTLV-III replication.
    Nature. 1986 May 22-28;321(6068):412-7 PMID: 3012355
  25. The role of mononuclear phagocytes in HTLV-III/LAV infection.
    Science. 1986 Jul 11;233(4760):215-9 PMID: 3014648
  26. HTLV-III expression and production involve complex regulation at the levels of splicing and translation of viral RNA.
    Cell. 1986 Sep 12;46(6):807-17 PMID: 3638988
  27. Polyomavirus small t antigen: overproduction in bacteria, purification, and utilization for monoclonal and polyclonal antibody production.
    J Virol. 1986 Dec;60(3):1075-84 PMID: 3023660
  28. Cloning of HTLV-4 and its relation to simian and human immunodeficiency viruses.
    Nature. 1987 Apr 9-15;326(6113):610-3 PMID: 3104797
  29. Genome organization and transactivation of the human immunodeficiency virus type 2.
    Nature. 1987 Apr 16-22;326(6114):662-9 PMID: 3031510
  30. Human immunodeficiency virus can productively infect cultured human glial cells.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3526-30 PMID: 3472222
  31. Sequence of simian immunodeficiency virus from macaque and its relationship to other human and simian retroviruses.
    Nature. 1987 Aug 6-12;328(6130):543-7 PMID: 3649576
  32. Human immunodeficiency virus: the eighth gene.
    AIDS Res Hum Retroviruses. 1987 Spring;3(1):33-9 PMID: 3476127
  33. Sequence of simian immunodeficiency virus from African green monkey, a new member of the HIV/SIV group.
    Nature. 1988 Jun 2;333(6172):457-61 PMID: 3374586
  34. Isolation and characterization of a novel protein (X-ORF product) from SIV and HIV-2.
    Science. 1988 Jul 8;241(4862):199-201 PMID: 3388031
  35. Identification of a novel retroviral gene unique to human immunodeficiency virus type 2 and simian immunodeficiency virus SIVMAC.
    J Virol. 1988 Sep;62(9):3501-5 PMID: 3136256
  36. Identification of a protein encoded by the vpu gene of HIV-1.
    Nature. 1988 Aug 11;334(6182):532-4 PMID: 3043230
  37. A naturally immunogenic virion-associated protein specific for HIV-2 and SIV.
    Nature. 1988 Sep 15;335(6187):262-5 PMID: 2842694
  38. Human immunodeficiency virus type 1 has an additional coding sequence in the central region of the genome.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6968-72 PMID: 2842797
  39. A novel gene of HIV-1, vpu, and its 16-kilodalton product.
    Science. 1988 Sep 2;241(4870):1221-3 PMID: 3261888
  40. Functional comparison of transactivation by simian immunodeficiency virus from rhesus macaques and human immunodeficiency virus type 1.
    J Virol. 1988 Dec;62(12):4523-32 PMID: 2846868
  41. Infection and replication of HIV-1 in purified progenitor cells of normal human bone marrow.
    Science. 1988 Nov 11;242(4880):919-22 PMID: 2460922
  42. Human immunodeficiency virus can infect CD4-negative human fibroblastoid cells.
    Proc Natl Acad Sci U S A. 1989 Jun;86(11):4287-90 PMID: 2786212
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.11.5688-5693.1990
Published
1990-11-00
Pages
5688-93
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC248631
Subset
IM
Grants
NCI NIH HHS · CA-39805 · United States
NHLBI NIH HHS · HL-33774 · United States
NHLBI NIH HHS · HL-43561 · United States
Databases
GENBANK
M60088
External Links
PubMed source DOI: 10.1128/JVI.64.11.5688-5693.1990 PMC Full Text View journal details
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