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PMID: 2304148 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

CD4-independent, productive infection of a neuronal cell line by human immunodeficiency virus type 1.

Journal of virology ·Vol. 64 ·No. 3 ·1990-03-00 ·Pages 1383-7

Li XL, Moudgil T, Vinters HV, Ho DD

Abstract

One neuronal cell line (SK-N-MC) was found to be susceptible to productive infection by multiple isolates of the human immunodeficiency virus type 1 (HIV-1). Characterization of SK-N-MC cells showed that these cells are neuroectodermal in origin in that they express dopamine hydroxylase, catecholamines, neuron-specific enolase, and neurofilaments. Despite their susceptibility to HIV-1 infection, SK-N-MC cells had no detectable CD4 and this infection was not blocked by anti-CD4 monoclonal antibodies (OKT4A, Leu3A) or recombinant soluble CD4. These experiments demonstrated that certain cells of neuroectodermal origin are susceptible to infection in vitro by HIV-1 via a CD4-independent mechanism.

MeSH Terms
Animals Antigens, CD/analysis Cell Line Cell Transformation, Viral HIV-1/genetics,physiology,ultrastructure Microscopy, Electron Neurons Tumor Cells, Cultured Virus Replication
Chemicals
Antigens, CD
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Li X L
Department of Medicine, Cedars-Sinai Medical Center and UCLA School of Medicine 90048.
Moudgil T
Vinters H V
Ho D D
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.3.1383-1387.1990
Published
1990-03-00
Pages
1383-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249262
Subset
IM
Grants
NIAID NIH HHS · AI25541 · United States
NIAID NIH HHS · AI28747 · United States
External Links
PubMed source DOI: 10.1128/JVI.64.3.1383-1387.1990 PMC Full Text View journal details
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