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PMID: 7708698 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Distinct functions of SR proteins in recruitment of U1 small nuclear ribonucleoprotein to alternative 5' splice sites.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 7 ·1995-03-28 ·Pages 2642-6

Zahler AM, Roth MB

Abstract

Alternative splicing of precursor messenger RNAs (pre-mRNAs) is an important mechanism for the regulation of gene expression. The members of the SR protein family of pre-mRNA splicing factors have distinct functions in promoting alternative splice site usage. Here we show that SR proteins are required for the first step of spliceosome assembly, interaction of the U1 small nuclear ribonucleoprotein complex (U1 snRNP) with the 5' splice site of the pre-mRNA. Further, we find that individual SR proteins have distinct abilities to promote interaction of U1 snRNP with alternative 5' splice junctions. These results suggest that SR proteins direct 5' splice site selection by regulation of U1 snRNP assembly onto the pre-mRNA.

MeSH Terms
Alternative Splicing Animals Antigens, Polyomavirus Transforming/biosynthesis Base Sequence Cattle DNA Primers Molecular Sequence Data Nuclear Proteins/isolation & purification,metabolism Phosphoproteins/isolation & purification,metabolism Polymerase Chain Reaction RNA Precursors/isolation & purification,metabolism RNA-Binding Proteins Ribonucleoprotein, U1 Small Nuclear/isolation & purification,metabolism Serine-Arginine Splicing Factors Simian virus 40/genetics Templates, Genetic Thymus Gland/metabolism
Chemicals
Antigens, Polyomavirus Transforming DNA Primers Nuclear Proteins Phosphoproteins RNA Precursors RNA-Binding Proteins Ribonucleoprotein, U1 Small Nuclear Serine-Arginine Splicing Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zahler A M
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA.
Roth M B
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.7.2642
Published
1995-03-28
Pages
2642-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC42274
Subset
IM
Grants
PHS HHS · 42786-02 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.7.2642 PMC Full Text View journal details
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