Alkaline phosphatase and peroxidase for double immunoenzymatic labelling of cellular constituents.

Journal of clinical pathology ·Vol. 31 ·No. 5 ·1978-05-00 ·Pages 454-60

Mason DY, Sammons R

Abstract

The use of alkaline phosphatase in an immunoenzymatic procedure for the localisation of antigens in paraffin sections or cell smears is described. The results of this method, when applied to the detection of immunoglobulins, lysozyme, or lactoferrin, were comparable in intensity and clarity to those obtained with the PAP immunoperoxidase procedure. Furthermore, double immunoenzymatic labelling (with alkaline phosphatase and peroxidase) of two cellular constituents in a tissue section is possible, the brown peroxidase reaction product contrasting well with the blue alkaline phosphatase product. Since the two antibody 'sandwiches' are applied simultaneously rather than sequentially the total duration of this double immunostaining procedure is only a few minutes longer than that required for detection of a single antigen. It was also found that the unlabelled antibody immunohistological procedure (whether used in conjunction with alkaline phosphatase or peroxidase) can be shortened without loss of sensitivity by carrying out two of the incubation steps simultaneously.

MeSH Terms

Alkaline Phosphatase Antigens/analysis Humans Immunoenzyme Techniques Peroxidases Staining and Labeling

Chemicals

Antigens Peroxidases Alkaline Phosphatase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Mason D Y

Sammons R

References (21)

21 references, click to expand

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Article Info

Journal

Journal of clinical pathology

Abbr.

J Clin Pathol

ISSN

0021-9746

DOI

10.1136/jcp.31.5.454

Published

1978-05-00

Pages

454-60

Language

English

Region

England

NLM ID

0376601

PMCID

PMC1145303

Subset

External Links

PubMed source DOI: 10.1136/jcp.31.5.454 PMC Full Text View journal details

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Alkaline phosphatase and peroxidase for double immunoenzymatic labelling of cellular constituents.

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