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PMID: 7739558 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yeast virus propagation depends critically on free 60S ribosomal subunit concentration.

Molecular and cellular biology ·Vol. 15 ·No. 5 ·1995-05-00 ·Pages 2772-81

Ohtake Y, Wickner RB

Abstract

Over 30 MAK (maintenance of killer) genes are necessary for propagation of the killer toxin-encoding M1 satellite double-stranded RNA of the L-A virus. Sequence analysis revealed that MAK7 is RPL4A, one of the two genes encoding ribosomal protein L4 of the 60S subunit. We further found that mutants with mutations in 18 MAK genes (including mak1 [top1], mak7 [rpl4A], mak8 [rpl3], mak11, and mak16) had decreased free 60S subunits. Mutants with another three mak mutations had half-mer polysomes, indicative of poor association of 60S and 40S subunits. The rest of the mak mutants, including the mak3 (N-acetyltransferase) mutant, showed a normal profile. The free 60S subunits, L-A copy number, and the amount of L-A coat protein in the mak1, mak7, mak11, and mak16 mutants were raised to the normal level by the respective normal single-copy gene. Our data suggest that most mak mutations affect M1 propagation by their effects on the supply of proteins from the L-A virus and that the translation of the non-poly(A) L-A mRNA depends critically on the amount of free 60S ribosomal subunits, probably because 60S association with the 40S subunit waiting at the initiator AUG is facilitated by the 3' poly(A).

Related Genes
MAK7 RPL4A
MeSH Terms
Genes, Fungal Models, Biological Molecular Sequence Data Mutation Protein Biosynthesis RNA Viruses/genetics,physiology RNA, Viral/genetics,metabolism Ribosomal Proteins/genetics Ribosomes/chemistry,metabolism Saccharomyces cerevisiae/genetics,metabolism,virology Saccharomyces cerevisiae Proteins/genetics Virus Replication
Chemicals
RNA, Viral Ribosomal Proteins Rpl3 protein, S cerevisiae Saccharomyces cerevisiae Proteins ribosomal protein L4
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ohtake Y
Section of Genetics of Simple Eukaryotes, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892, USA.
Wickner R B
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.5.2772
Published
1995-05-00
Pages
2772-81
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230508
Subset
IM
Databases
GENBANK
U17361
External Links
PubMed source DOI: 10.1128/MCB.15.5.2772 PMC Full Text View journal details
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