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PMID: 7745723 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A subpopulation of normal B cells latently infected with Epstein-Barr virus resembles Burkitt lymphoma cells in expressing EBNA-1 but not EBNA-2 or LMP1.

Journal of virology ·Vol. 69 ·No. 6 ·1995-06-00 ·Pages 3752-8

Chen F, Zou JZ, di Renzo L, Winberg G, Hu LF, Klein E, Klein G, Ernberg I

Abstract

Using reverse transcription of whole cellular RNA and nested PCR, we have performed experiments mixing different proportions of Epstein-Barr virus (EBV)-carrying and EBV-negative cells. Based on the results, a method that detects viral transcripts for EBNA-1, EBNA-2, LMP1, and LMP2a from less than one positive cell among 10(5) negative cells was developed. With this method we have shown that the EBV DNA positive cells among small, high-density peripheral blood B-lymphocytes of normal healthy persons express EBNA-1-mRNA but not EBNA-2 or LMP1. A similar EBV expression pattern is found in type I Burkitt lymphoma cells. We suggest that the expression pattern in the lymphoma cells reflects the viral strategy in normal resting B cells and meets the requirements of latent persistence.

MeSH Terms
Animals Antigens, Viral/biosynthesis,genetics B-Lymphocytes/immunology,metabolism,virology Base Sequence Burkitt Lymphoma/immunology,metabolism,pathology Callithrix Cell Line DNA Primers DNA-Binding Proteins/biosynthesis,genetics Epstein-Barr Virus Nuclear Antigens Herpesvirus 4, Human/immunology,physiology Humans Molecular Sequence Data Tumor Cells, Cultured Viral Matrix Proteins/biosynthesis,genetics Virus Latency
Chemicals
Antigens, Viral DNA Primers DNA-Binding Proteins EBV-associated membrane antigen, Epstein-Barr virus Epstein-Barr Virus Nuclear Antigens Viral Matrix Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Chen F
Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden.
Zou J Z
di Renzo L
Winberg G
Hu L F
Klein E
Klein G
Ernberg I
References (41)
41 references, click to expand
  1. The establishment of lymphoblastoid lines from adult and fetal human lymphoid tissue and its dependence on EBV.
    Int J Cancer. 1971 Nov 15;8(3):443-50 PMID: 4332899
  2. Eradication of Epstein-Barr virus by allogeneic bone marrow transplantation: implications for sites of viral latency.
    Proc Natl Acad Sci U S A. 1988 Nov;85(22):8693-6 PMID: 2847171
  3. One of two Epstein-Barr virus nuclear antigens contains a glycine-alanine copolymer domain.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5665-9 PMID: 6310587
  4. A membrane protein encoded by Epstein-Barr virus in latent growth-transforming infection.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7207-11 PMID: 6095274
  5. Defective regulation of Epstein-Barr virus infection in patients with acquired immunodeficiency syndrome (AIDS) or AIDS-related disorders.
    N Engl J Med. 1986 Apr 3;314(14):874-9 PMID: 3005862
  6. Antigen-driven selection of virgin and memory B cells.
    Immunol Rev. 1986 Jun;91:61-85 PMID: 3089914
  7. Characterization of EBV-carrying B-cell populations in healthy seropositive individuals with regard to density, release of transforming virus and spontaneous outgrowth.
    Int J Cancer. 1987 Apr 15;39(4):472-6 PMID: 3030940
  8. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  9. A promoter for the highly spliced EBNA family of RNAs of Epstein-Barr virus.
    J Virol. 1987 Nov;61(11):3424-30 PMID: 2822952
  10. Interaction of the lymphocyte-derived Epstein-Barr virus nuclear antigen EBNA-1 with its DNA-binding sites.
    J Virol. 1989 Jan;63(1):101-10 PMID: 2535719
  11. Epstein-Barr virus-infected B cells persist in the circulation of acyclovir-treated virus carriers.
    Int J Cancer. 1989 Jan 15;43(1):67-71 PMID: 2536009
  12. Host cell-dependent regulation of growth transformation-associated Epstein-Barr virus antigens in somatic cell hybrids.
    J Virol. 1989 Jun;63(6):2768-72 PMID: 2542588
  13. Viral latency and transformation: the strategy of Epstein-Barr virus.
    Cell. 1989 Jul 14;58(1):5-8 PMID: 2546674
  14. Mutually exclusive use of viral promoters in Epstein-Barr virus latently infected lymphocytes.
    Proc Natl Acad Sci U S A. 1989 Sep;86(17):6498-502 PMID: 2549539
  15. Mechanism of antigen-driven selection in germinal centres.
    Nature. 1989 Dec 21-28;342(6252):929-31 PMID: 2594086
  16. Different Epstein-Barr virus-B cell interactions in phenotypically distinct clones of a Burkitt's lymphoma cell line.
    J Gen Virol. 1990 Jul;71 ( Pt 7):1481-95 PMID: 2165133
  17. Epstein-Barr virus-associated peripheral T-cell lymphoma of activated CD8 phenotype.
    Cancer. 1990 Dec 15;66(12):2557-62 PMID: 2174302
  18. Infection of human thymocytes by Epstein-Barr virus.
    J Exp Med. 1991 Apr 1;173(4):971-80 PMID: 1706754
  19. Differential expression of Epstein Barr viral transcripts for two proteins (TP1 and LMP) in lymphocyte and epithelial cells.
    Nucleic Acids Res. 1991 May 11;19(9):2435-40 PMID: 1710355
  20. Epstein-Barr virus latent membrane protein expression in Hodgkin and Reed-Sternberg cells.
    Proc Natl Acad Sci U S A. 1991 Jun 1;88(11):4766-70 PMID: 1647016
  21. Restricted Epstein-Barr virus protein expression in Burkitt lymphoma is due to a different Epstein-Barr nuclear antigen 1 transcriptional initiation site.
    Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6343-7 PMID: 1648738
  22. Exclusive expression of Epstein-Barr virus nuclear antigen 1 in Burkitt lymphoma arises from a third promoter, distinct from the promoters used in latently infected lymphocytes.
    Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6550-4 PMID: 1713681
  23. Three pathways of Epstein-Barr virus gene activation from EBNA1-positive latency in B lymphocytes.
    J Virol. 1992 Jan;66(1):122-31 PMID: 1309242
  24. B cell phenotype-dependent expression of the Epstein-Barr virus nuclear antigens EBNA-2 to EBNA-6: studies with somatic cell hybrids.
    J Gen Virol. 1991 Dec;72 ( Pt 12):3025-33 PMID: 1662694
  25. Host-cell-phenotype-dependent control of the BCR2/BWR1 promoter complex regulates the expression of Epstein-Barr virus nuclear antigens 2-6.
    Proc Natl Acad Sci U S A. 1992 Feb 1;89(3):905-9 PMID: 1371012
  26. Three transcriptionally distinct forms of Epstein-Barr virus latency in somatic cell hybrids: cell phenotype dependence of virus promoter usage.
    Virology. 1992 Mar;187(1):189-201 PMID: 1310556
  27. Epstein-Barr virus latent gene expression in uncultured peripheral blood lymphocytes.
    J Virol. 1992 Jun;66(6):3715-24 PMID: 1316478
  28. Identification of target antigens for the human cytotoxic T cell response to Epstein-Barr virus (EBV): implications for the immune control of EBV-positive malignancies.
    J Exp Med. 1992 Jul 1;176(1):157-68 PMID: 1319456
  29. Localization of Epstein-Barr virus cytotoxic T cell epitopes using recombinant vaccinia: implications for vaccine development.
    J Exp Med. 1992 Jul 1;176(1):169-76 PMID: 1377222
  30. The Epstein-Barr virus nuclear protein 1 promoter active in type I latency is autoregulated.
    J Virol. 1992 Aug;66(8):4654-61 PMID: 1321268
  31. Recognition of the Epstein-Barr virus-encoded nuclear antigens EBNA-4 and EBNA-6 by HLA-A11-restricted cytotoxic T lymphocytes: implications for down-regulation of HLA-A11 in Burkitt lymphoma.
    Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5862-6 PMID: 1321426
  32. The Epstein-Barr virus (EBV) nuclear antigen 1 BamHI F promoter is activated on entry of EBV-transformed B cells into the lytic cycle.
    J Virol. 1992 Dec;66(12):7461-8 PMID: 1331531
  33. Multiple HLA A11-restricted cytotoxic T-lymphocyte epitopes of different immunogenicities in the Epstein-Barr virus-encoded nuclear antigen 4.
    J Virol. 1993 Mar;67(3):1572-8 PMID: 7679748
  34. Clonality, expression and methylation patterns of the Epstein-Barr virus genomes in lethal midline granulomas classified as peripheral angiocentric T cell lymphomas.
    J Gen Virol. 1994 Jan;75 ( Pt 1):77-84 PMID: 8113742
  35. Detection of multiple 'Ebnotypes' in individual Epstein-Barr virus carriers following lymphocyte transformation by virus derived from peripheral blood and oropharynx.
    J Gen Virol. 1994 Jan;75 ( Pt 1):85-94 PMID: 8113743
  36. Epstein-Barr virus latency in blood mononuclear cells: analysis of viral gene transcription during primary infection and in the carrier state.
    J Virol. 1994 Nov;68(11):7374-85 PMID: 7933121
  37. Differences in B cell growth phenotype reflect novel patterns of Epstein-Barr virus latent gene expression in Burkitt's lymphoma cells.
    EMBO J. 1987 Sep;6(9):2743-51 PMID: 2824192
  38. The Epstein-Barr virus proteins.
    Adv Cancer Res. 1988;50:95-158 PMID: 2837072
  39. A spliced Epstein-Barr virus gene expressed in immortalized lymphocytes is created by circularization of the linear viral genome.
    EMBO J. 1988 Mar;7(3):769-74 PMID: 2840285
  40. Expression of Epstein-Barr virus-encoded proteins in nasopharyngeal carcinoma.
    Int J Cancer. 1988 Sep 15;42(3):329-38 PMID: 2843473
  41. Quantitative evaluation of Epstein-Barr-virus-infected mononuclear peripheral blood leukocytes in infectious mononucleosis.
    N Engl J Med. 1977 Jan 20;296(3):132-4 PMID: 187934
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.69.6.3752-3758.1995
Published
1995-06-00
Pages
3752-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC189092
Subset
IM
Grants
NCI NIH HHS · 5 ROI CA 30264-12 · United States
External Links
PubMed source DOI: 10.1128/JVI.69.6.3752-3758.1995 PMC Full Text View journal details
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