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PMID: 7753843 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interferons alpha and beta down-regulate the expression of basic fibroblast growth factor in human carcinomas.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 10 ·1995-05-09 ·Pages 4562-6

Singh RK, Gutman M, Bucana CD, Sanchez R, Llansa N, Fidler IJ

Abstract

We investigated the influence of interferons alpha, beta, and gamma (IFN-alpha, -beta, and -gamma) on the production of basic fibroblast growth factor (bFGF) by human renal carcinoma cells. The human renal carcinoma cell metastatic line SN12PM6 was established in culture from a lung metastasis and SN12PM6-resistant cells were selected in vitro for resistance to the antiproliferative effects of IFN-alpha or IFN-beta. IFN-alpha and IFN-beta, but not IFN-gamma, down-regulated the expression of bFGF at the mRNA and protein levels by a mechanism independent of their antiproliferative effects. Down-regulation of bFGF required a long exposure (> 4 days) of cells to low concentrations (> 10 units/ml) of IFN-alpha or IFN-beta. The withdrawal of IFN-alpha or IFN-beta from the medium permitted SN12PM6-resistant cells to resume production of bFGF. The incubation of human bladder, prostate, colon, and breast carcinoma cells with noncytostatic concentrations of IFN-alpha or IFN-beta also produced down-regulation of bFGF production.

MeSH Terms
Base Sequence Blotting, Northern Carcinoma, Renal Cell/secondary Cell Division/drug effects Cell Line Fibroblast Growth Factor 2/biosynthesis Gene Expression Regulation, Neoplastic/drug effects Humans In Situ Hybridization Interferon alpha-2 Interferon-alpha/pharmacology Interferon-beta/pharmacology Kidney Neoplasms Kinetics Lung Neoplasms/secondary Molecular Sequence Data Oligonucleotide Probes RNA, Messenger/analysis,biosynthesis Recombinant Proteins Time Factors Tumor Cells, Cultured
Chemicals
Interferon alpha-2 Interferon-alpha Oligonucleotide Probes RNA, Messenger Recombinant Proteins Fibroblast Growth Factor 2 Interferon-beta
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Singh R K
Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
Gutman M
Bucana C D
Sanchez R
Llansa N
Fidler I J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.10.4562
Published
1995-05-09
Pages
4562-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41984
Subset
IM
Grants
NCI NIH HHS · CA 16672 · United States
NCI NIH HHS · R35-CA 42107 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.10.4562 PMC Full Text View journal details
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