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PMID: 7760804 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

E2F-5, a new E2F family member that interacts with p130 in vivo.

Molecular and cellular biology ·Vol. 15 ·No. 6 ·1995-06-00 ·Pages 3082-9

Hijmans EM, Voorhoeve PM, Beijersbergen RL, van 't Veer LJ, Bernards R

Abstract

E2F DNA binding sites are found in a number of genes whose expression is tightly regulated during the cell cycle. The activity of E2F transcription factors is regulated by association with specific repressor molecules that can bind and inhibit the E2F transactivation domain. For E2F-1, E2F-2, and E2F-3, the repressor is the product of the retinoblastoma gene, pRb. E2f-4 interacts with pRb-related p107 and not with pRb itself. Recently, a cDNA encoding a third member of the retinoblastoma gene family, p130, was isolated. p130 also interacts with E2F DNA binding activity, primarily in the G0 phase of the cell cycle. We report here the cloning of a fifth member of the E2F gene family. The human E2F-5 cDNA encodes a 346-amino-acid protein with a predicted molecular mass of 38 kDa. E2F-5 is more closely related to E2F-4 (78% similarity) than to E2F-1 (57% similarity). E2F-5 resembles the other E2Fs in that it binds to a consensus E2F site in a cooperative fashion with DP-1. By using a specific E2F-5 antiserum, we found that under physiological conditions, E2F-5 interacts preferentially with p130.

MeSH Terms
Adenovirus E2 Proteins/genetics,isolation & purification,metabolism Amino Acid Sequence Base Sequence Cell Line Cloning, Molecular E2F5 Transcription Factor Genes, Retinoblastoma/genetics Humans Molecular Sequence Data Retinoblastoma Protein/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Transcription Factors/genetics,isolation & purification,metabolism
Chemicals
Adenovirus E2 Proteins E2F5 Transcription Factor E2F5 protein, human Retinoblastoma Protein Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hijmans E M
Division of Molecular Carcinogenesis, Netherlands Cancer Institute, Amsterdam.
Voorhoeve P M
Beijersbergen R L
van 't Veer L J
Bernards R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.6.3082
Published
1995-06-00
Pages
3082-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230539
Subset
IM
Databases
GENBANK
X86097
External Links
PubMed source DOI: 10.1128/MCB.15.6.3082 PMC Full Text View journal details
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