Enzyme-linked immunosorbent assay for streptococcal M protein antibodies.

Journal of clinical microbiology ·Vol. 3 ·No. 5 ·1976-05-00 ·Pages 501-5

Russell H, Facklam RR, Edwards LR

Abstract

The enzyme-linked immunosorbent assay (ELISA) described by Engvall and Perlmann, which uses antigen-coated tubes and enzyme-labeled anti-immunoglobulin, has been used for the detection of antibodies against streptococcal M protein. The antigen used in the assay was obtained by guanidine extraction of type M-12 streptococcal cell walls followed by hydroxyapatite chromatography. This antigen has the capacity to elicit bactericidal antibodies in rabbits. The results show that the ELISA is specific and highly sensitive for the detection of antibodies in rabbit and human antisera. Preliminary results suggest that, when M-12 antigen is used, the antibodies detected by ELISA are the same antibodies detected in the bactericidal test. The assay has been performed with human and rabbit sera. There was a 96% agreement between bactericidal and ELISA results with rabbit sera and 97.5% agreement with human sera. All bactericidal antibody-positive sera tested thus far yielded positive ELISA results.

MeSH Terms

Alkaline Phosphatase Animals Antibodies, Bacterial/analysis Antigens, Bacterial Bacterial Proteins/immunology Blood Bactericidal Activity Humans Immunoglobulins Immunologic Techniques Rabbits/immunology Streptococcus pyogenes/immunology

Chemicals

Antibodies, Bacterial Antigens, Bacterial Bacterial Proteins Immunoglobulins Alkaline Phosphatase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Russell H

Facklam R R

Edwards L R

References (23)

23 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.3.5.501-505.1976

Published

1976-05-00

Pages

501-5

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC274338

Subset

External Links

PubMed source DOI: 10.1128/jcm.3.5.501-505.1976 PMC Full Text View journal details

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Enzyme-linked immunosorbent assay for streptococcal M protein antibodies.

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