Home LiteratureArticle Details
PMID: 7806575 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

STU1, a suppressor of a beta-tubulin mutation, encodes a novel and essential component of the yeast mitotic spindle.

The Journal of cell biology ·Vol. 127 ·No. 6 Pt 2 ·1994-12-00 ·Pages 1973-84

Pasqualone D, Huffaker TC

Abstract

We have isolated a cold-sensitive allele of TUB2, the sole gene encoding beta-tubulin in S. cerevisiae, that confers a specific defect in spindle microtubule function. At 14 degrees C, tub2-406 cells lack a normal bipolar spindle but do assemble functional cytoplasmic microtubules. In an attempt to identify proteins that are important for spindle assembly, we screened for suppressors of the cold-sensitivity of tub2-406 and obtained four alleles of a novel gene, STU1. Genetic interactions between stu1 alleles and alleles of TUB1 and TUB2 suggest that Stu1p specifically interacts with microtubules. STU1 is essential for growth and disruption of STU1 causes defects in spindle assembly that are similar to those produced by the tub2-406 mutation. The nucleotide sequence of the STU1 gene predicts a protein product of 174 kD with no significant similarity to known proteins. An epitope-tagged Stulp colocalizes with microtubules in the mitotic spindle of yeast. These results demonstrate that Stulp is an essential component of the yeast mitotic spindle.

Related Genes
STU1 TUB2
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Cold Temperature Fluorescent Antibody Technique Genes, Fungal Microtubule-Associated Proteins/genetics,immunology,isolation & purification,metabolism Microtubules/metabolism Molecular Sequence Data Mutation Protein Binding Saccharomyces cerevisiae/genetics,growth & development,ultrastructure Saccharomyces cerevisiae Proteins Sequence Analysis, DNA Spindle Apparatus/chemistry,physiology,ultrastructure Suppression, Genetic Tubulin/genetics,metabolism
Chemicals
Microtubule-Associated Proteins STU1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Tubulin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pasqualone D
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853.
Huffaker T C
References (38)
38 references, click to expand
  1. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6 kilobase pairs.
    Genetics. 1993 May;134(1):81-150 PMID: 8514151
  2. Suppression of a myosin defect by a kinesin-related gene.
    Nature. 1992 Mar 26;356(6367):358-61 PMID: 1549181
  3. Disruption of mitotic spindle orientation in a yeast dynein mutant.
    Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10096-100 PMID: 8234262
  4. Cytoplasmic dynein is required for normal nuclear segregation in yeast.
    Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11172-6 PMID: 8248224
  5. Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein.
    J Cell Biol. 1994 Feb;124(4):507-19 PMID: 8106549
  6. Systematic mutational analysis of the yeast beta-tubulin gene.
    Mol Biol Cell. 1994 Jan;5(1):29-43 PMID: 8186463
  7. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.
    J Cell Biol. 1982 Jun;93(3):576-82 PMID: 6811596
  8. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  9. Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces.
    J Cell Biol. 1984 Mar;98(3):922-33 PMID: 6365930
  10. The structure of an antigenic determinant in a protein.
    Cell. 1984 Jul;37(3):767-78 PMID: 6204768
  11. Isolation of the beta-tubulin gene from yeast and demonstration of its essential function in vivo.
    Cell. 1983 May;33(1):211-9 PMID: 6380751
  12. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.
    Mol Gen Genet. 1984;197(2):345-6 PMID: 6394957
  13. Yeast proteins associated with microtubules in vitro and in vivo.
    Mol Biol Cell. 1992 Jan;3(1):29-47 PMID: 1348005
  14. Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly.
    J Cell Biol. 1992 Jul;118(1):109-20 PMID: 1618897
  15. Kinesin-related proteins required for assembly of the mitotic spindle.
    J Cell Biol. 1992 Jul;118(1):95-108 PMID: 1618910
  16. Kinesin-related proteins required for structural integrity of the mitotic spindle.
    Cell. 1992 Aug 7;70(3):451-8 PMID: 1643659
  17. CIK1: a developmentally regulated spindle pole body-associated protein important for microtubule functions in Saccharomyces cerevisiae.
    Genes Dev. 1992 Aug;6(8):1414-29 PMID: 1644287
  18. Astral microtubules are not required for anaphase B in Saccharomyces cerevisiae.
    J Cell Biol. 1992 Oct;119(2):379-88 PMID: 1400581
  19. NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae.
    J Cell Biol. 1993 May;121(3):503-12 PMID: 8486732
  20. Isolation and characterization of mutations in the beta-tubulin gene of Saccharomyces cerevisiae.
    Genetics. 1985 Dec;111(4):715-34 PMID: 2998923
  21. A chicken-yeast chimeric beta-tubulin protein is incorporated into mouse microtubules in vivo.
    Cell. 1986 Feb 14;44(3):461-8 PMID: 3753663
  22. Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins.
    Mol Cell Biol. 1986 Nov;6(11):3711-21 PMID: 3025610
  23. Genetic analysis of the yeast cytoskeleton.
    Annu Rev Genet. 1987;21:259-84 PMID: 3327466
  24. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector.
    Gene. 1987;60(2-3):237-43 PMID: 3327750
  25. Improved tools for biological sequence comparison.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 PMID: 3162770
  26. Diverse effects of beta-tubulin mutations on microtubule formation and function.
    J Cell Biol. 1988 Jun;106(6):1997-2010 PMID: 3290223
  27. Unlinked noncomplementation: isolation of new conditional-lethal mutations in each of the tubulin genes of Saccharomyces cerevisiae.
    Genetics. 1988 Jun;119(2):249-60 PMID: 3294100
  28. Functions of microtubules in the Saccharomyces cerevisiae cell cycle.
    J Cell Biol. 1988 Oct;107(4):1409-26 PMID: 3049620
  29. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  30. KAR3, a kinesin-related gene required for yeast nuclear fusion.
    Cell. 1990 Mar 23;60(6):1029-41 PMID: 2138512
  31. Components of the yeast spindle and spindle pole body.
    J Cell Biol. 1990 Nov;111(5 Pt 1):1913-27 PMID: 2229181
  32. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  33. BIK1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae, colocalizes with tubulin.
    J Cell Biol. 1990 Dec;111(6 Pt 1):2573-86 PMID: 2277073
  34. Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.
    Methods Enzymol. 1991;194:281-301 PMID: 2005793
  35. Getting started with yeast.
    Methods Enzymol. 1991;194:3-21 PMID: 2005794
  36. Preparation of yeast cells for thin-section electron microscopy.
    Methods Enzymol. 1991;194:602-8 PMID: 2005811
  37. Multifunctional yeast high-copy-number shuttle vectors.
    Gene. 1992 Jan 2;110(1):119-22 PMID: 1544568
  38. Loss of function of Saccharomyces cerevisiae kinesin-related CIN8 and KIP1 is suppressed by KAR3 motor domain mutations.
    Genetics. 1993 Sep;135(1):35-44 PMID: 8224825
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.127.6.1973
Published
1994-12-00
Pages
1973-84
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120270
Subset
IM
Grants
NIGMS NIH HHS · R01 GM040479 · United States
NIGMS NIH HHS · GM40479 · United States
Databases
GENBANK
Z35655
External Links
PubMed source DOI: 10.1083/jcb.127.6.1973 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2