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PMID: 7864829 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Sheep pancreatic microsomes as an alternative to the dog source for studying protein translocation.

The Biochemical journal ·Vol. 306 ( Pt 1) ·1995-02-15 ·Pages 57-61

Kaderbhai MA, Harding VJ, Karim A, Austen BM, Kaderbhai NN

Abstract

A procedure is described for the preparation of rough membrane vesicles of endoplasmic-reticular origin from the pancreas of sheep. These isolated membranes translocate, process and glycosylate in vitro-translated heterologous proteins in a manner comparable with that exhibited by dog pancreatic microsomes.

MeSH Terms
Animals Biological Transport Dogs Endopeptidases/metabolism Endoplasmic Reticulum/metabolism,ultrastructure Escherichia coli/enzymology Intracellular Membranes/metabolism,ultrastructure Mating Factor Membrane Proteins Microscopy, Electron Microsomes/metabolism Pancreas/ultrastructure Peptides/metabolism Protein Precursors/metabolism Protein Sorting Signals/metabolism,pharmacology Proteins/metabolism Saccharomyces cerevisiae/chemistry Serine Endopeptidases Sheep beta-Lactamases/metabolism
Chemicals
Membrane Proteins Peptides Protein Precursors Protein Sorting Signals Proteins Mating Factor Endopeptidases Serine Endopeptidases type I signal peptidase beta-Lactamases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kaderbhai M A
Institute of Biological Sciences, University of Wales, Penglais, Aberystwyth, U.K.
Harding V J
Karim A
Austen B M
Kaderbhai N N
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj3060057
Published
1995-02-15
Pages
57-61
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1136481
Subset
IM
External Links
PubMed source DOI: 10.1042/bj3060057 PMC Full Text View journal details
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