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PMID: 7869025 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The B cell antigen receptor of class IgD induces a stronger and more prolonged protein tyrosine phosphorylation than that of class IgM.

The Journal of experimental medicine ·Vol. 181 ·No. 3 ·1995-03-01 ·Pages 1005-14

Kim KM, Reth M

Abstract

Most mature B lymphocytes coexpress two classes of antigen receptor, immunoglobulin (Ig)M and IgD. The differences in the signal transduction from the two receptors are still a matter of controversy. We have analyzed B cell lines expressing IgM or IgD antigen receptors with the same antigen specificity. Cross-linking of these receptors with either antigen, or class-specific antibodies, results in the activation of protein tyrosine kinases and the phosphorylation of the same substrate proteins. The kinetic and the intensity of phosphorylation, however, was quite different between the two receptors when they were cross-linked by antigen. In membrane IgM-expressing cells, the substrate phosphorylation reached a maximum after 1 minute and diminished after 60 minutes whereas, in the membrane IgD-expressing cells, the substrate phosphorylation increased further over time, reached its maximum at 60 minutes, and persisted longer than 240 minutes after exposure to antigen. As a result, the intensity of protein tyrosine phosphorylation induced by cross-linking of membrane IgD was stronger than that induced by membrane IgM. Studies of chimeric receptors demonstrate that only the membrane-proximal C domain and/or the transmembrane part of membrane-bound IgD molecule is required for the long-lasting substrate phosphorylation. Together, these data suggest that the signal emission from the two receptors is controlled differently.

MeSH Terms
Antibodies, Anti-Idiotypic/physiology Base Sequence Humans Immunoglobulin M/physiology Molecular Sequence Data Phosphorylation Protein-Tyrosine Kinases/metabolism Receptors, Antigen, B-Cell/physiology Receptors, Nerve Growth Factor/metabolism Tumor Cells, Cultured Tyrosine/metabolism
Chemicals
Antibodies, Anti-Idiotypic Immunoglobulin M Receptors, Antigen, B-Cell Receptors, Nerve Growth Factor Tyrosine Protein-Tyrosine Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kim K M
Max-Planck Institut für Immunbiologie, Freiburg, Germany.
Reth M
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.181.3.1005
Published
1995-03-01
Pages
1005-14
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2191939
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.181.3.1005 PMC Full Text View journal details
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