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PMID: 8070406 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The IgM antigen receptor of B lymphocytes is associated with prohibitin and a prohibitin-related protein.

The EMBO journal ·Vol. 13 ·No. 16 ·1994-08-15 ·Pages 3782-92

Terashima M, Kim KM, Adachi T, Nielsen PJ, Reth M, Köhler G, Lamers MC

Abstract

The two major classes of antigen receptors on murine B lymphocytes, mIgM and mIgD, are both contained in a complex with two additional molecules, Ig-alpha and Ig-beta, which permit signal transduction. Accordingly, early biochemical events after antigen binding to either receptor are similar; biological effects, however, are different. Here, we describe three newly discovered intracellular proteins of 32, 37 and 41 kDa molecular mass, that are non-covalently associated with mIgM, but not with mIgD. These proteins coprecipitate with mIgM in Triton X-100 and Nonidet P-40, but not in digitonin lysates. In addition, mIgM is to some extent associated with 29 and 31 kDa proteins that are predominantly associated with mIgD (see accompanying paper). Amino acid sequencing of p32 and p37 identified p32 as mouse prohibitin; this was corroborated by Western blot analysis with antibodies specific for rat prohibitin. p37 is a newly discovered protein. cDNA clones for both proteins were isolated and sequenced. The deduced amino acid sequence of p32 is identical to that of rat prohibitin. p37 is highly homologous to p32. Since prohibitin was identified as an inhibitor of cell proliferation, its association with mIgM, but not mIgD, could explain the different biological events elicited after engagement of each receptor.

MeSH Terms
Amino Acid Sequence Animals B-Lymphocytes/immunology Base Sequence Cloning, Molecular Immunoglobulin D/analysis Immunoglobulin M/analysis Mice Mice, Inbred C57BL Molecular Sequence Data Prohibitins Proteins/analysis,genetics Receptors, Antigen, B-Cell/analysis,chemistry Repressor Proteins Sequence Analysis Sequence Homology, Amino Acid Spleen/cytology
Chemicals
Immunoglobulin D Immunoglobulin M Prohibitins Proteins Receptors, Antigen, B-Cell Repressor Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Terashima M
Max-Planck-Institut für Immunobiologie, Freiburg, Germany.
Kim K M
Adachi T
Nielsen P J
Reth M
Köhler G
Lamers M C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-08-15
Pages
3782-92
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395291
Subset
IM
Databases
GENBANK
X78682, X78683, X78684
External Links
PubMed source PMC Full Text View journal details
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