Home LiteratureArticle Details
PMID: 7892240 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Synaptotagmin IV is an immediate early gene induced by depolarization in PC12 cells and in brain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 6 ·1995-03-14 ·Pages 2164-8

Vician L, Lim IK, Ferguson G, Tocco G, Baudry M, Herschman HR

Abstract

Subtractive library construction and differential screening were used to identify a cDNA for a cell type-specific immediate early gene induced in rat PC12 pheochromocytoma cells. Sequencing identified the protein product of this gene as rat synaptotagmin IV (SytIV). Synaptotagmins are synaptic vesicle proteins thought to play a role in depolarization-induced, calcium-mediated exocytosis and neurotransmitter release. SytIV mRNA accumulation is transiently induced in PC12 cells by potassium depolarization, calcium ionophore, ATP, and forskolin. In contrast, growth factors and phorbol 12-myristate 13-acetate induce little or no SytIV mRNA accumulation. Kainic acid-induced seizures in rats are followed by accumulation of SytIV message in the hippocampus and piriform cortex. The SytIV gene may provide a direct link between depolarization-induced neuronal gene expression and subsequent modulation of synaptic structure and function.

MeSH Terms
Adrenal Gland Neoplasms Amino Acid Sequence Animals Base Sequence Brain/drug effects,metabolism Calcium-Binding Proteins Cell Line Colforsin/pharmacology DNA Primers Gene Expression/drug effects Gene Library Genes, Immediate-Early Hippocampus/metabolism Kainic Acid/pharmacology Male Membrane Glycoproteins/biosynthesis,genetics Membrane Potentials Mice Molecular Sequence Data Nerve Tissue Proteins/biosynthesis,genetics Oligonucleotide Probes Organ Specificity PC12 Cells Pheochromocytoma Polymerase Chain Reaction Potassium/pharmacology RNA, Messenger/analysis,biosynthesis Rats Rats, Sprague-Dawley Seizures/chemically induced,metabolism Sequence Homology, Amino Acid Synaptotagmins Tetradecanoylphorbol Acetate/pharmacology
Chemicals
Calcium-Binding Proteins DNA Primers Membrane Glycoproteins Nerve Tissue Proteins Oligonucleotide Probes RNA, Messenger Syt4 protein, mouse Syt4 protein, rat Synaptotagmins Colforsin Tetradecanoylphorbol Acetate Potassium Kainic Acid
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Vician L
Department of Biological Chemistry, University of California, Los Angeles 90024.
Lim I K
Ferguson G
Tocco G
Baudry M
Herschman H R
References (31)
31 references, click to expand
  1. Domain structure of synaptotagmin (p65)
    J Biol Chem. 1991 Jan 5;266(1):623-9 PMID: 1985919
  2. Simple and efficient subtractive hybridization screening.
    Biotechniques. 1991 Nov;11(5):598, 600, 602-4 PMID: 1687108
  3. Temporal lobe epilepsy of the rat: differential expression of mRNAs of chromogranin B, secretogranin II, synaptin/synaptophysin and p65 in subfield of the hippocampus.
    Brain Res Mol Brain Res. 1992 Nov;16(1-2):1-12 PMID: 1334187
  4. Tissue-plasminogen activator is induced as an immediate-early gene during seizure, kindling and long-term potentiation.
    Nature. 1993 Feb 4;361(6411):453-7 PMID: 8429885
  5. Inhibition of neurotransmitter release by C2-domain peptides implicates synaptotagmin in exocytosis.
    Nature. 1993 May 13;363(6425):163-5 PMID: 8097867
  6. Numerous candidate plasticity-related genes revealed by differential cDNA cloning.
    Nature. 1993 Jun 24;363(6431):718-22 PMID: 8515813
  7. Synaptic transmission persists in synaptotagmin mutants of Drosophila.
    Cell. 1993 Jul 2;73(7):1281-90 PMID: 8100740
  8. Synaptic function is impaired but not eliminated in C. elegans mutants lacking synaptotagmin.
    Cell. 1993 Jul 2;73(7):1291-305 PMID: 8391930
  9. Expression of a mitogen-inducible cyclooxygenase in brain neurons: regulation by synaptic activity and glucocorticoids.
    Neuron. 1993 Aug;11(2):371-86 PMID: 8352945
  10. Synaptotagmin: a membrane constituent of neuropeptide-containing large dense-core vesicles.
    J Neurosci. 1993 Sep;13(9):3895-903 PMID: 8366350
  11. Mutational analysis of Drosophila synaptotagmin demonstrates its essential role in Ca(2+)-activated neurotransmitter release.
    Cell. 1993 Sep 24;74(6):1125-34 PMID: 8104705
  12. A single C2 domain from synaptotagmin I is sufficient for high affinity Ca2+/phospholipid binding.
    J Biol Chem. 1993 Dec 15;268(35):26386-90 PMID: 8253763
  13. Calcium-dependent interaction of the cytoplasmic region of synaptotagmin with membranes. Autonomous function of a single C2-homologous domain.
    J Biol Chem. 1994 Feb 25;269(8):5735-41 PMID: 8119912
  14. Bovine posterior pituitary: presence of p65 (synaptotagmin), PC1, PC2 and secretoneurin in large dense core vesicles.
    Neuroendocrinology. 1994 Feb;59(2):169-75 PMID: 8127407
  15. The COOH terminus of synaptotagmin mediates interaction with the neurexins.
    J Biol Chem. 1994 Mar 18;269(11):8576-81 PMID: 8132583
  16. The effect on synaptic physiology of synaptotagmin mutations in Drosophila.
    Neuron. 1994 Apr;12(4):909-20 PMID: 7909234
  17. Synaptotagmin III is a novel isoform of rat synaptotagmin expressed in endocrine and neuronal cells.
    J Biol Chem. 1994 Apr 22;269(16):11675-8 PMID: 8163462
  18. rheb, a growth factor- and synaptic activity-regulated gene, encodes a novel Ras-related protein.
    J Biol Chem. 1994 Jun 10;269(23):16333-9 PMID: 8206940
  19. A third synaptotagmin gene, Syt3, in the mouse.
    Proc Natl Acad Sci U S A. 1994 Aug 16;91(17):8195-9 PMID: 8058779
  20. A method for isolation of intact, translationally active ribonucleic acid.
    DNA. 1983;2(4):329-35 PMID: 6198133
  21. Analysis of membrane and surface protein sequences with the hydrophobic moment plot.
    J Mol Biol. 1984 Oct 15;179(1):125-42 PMID: 6502707
  22. Molecular cloning of a gene sequence regulated by nerve growth factor.
    Science. 1985 Jul 26;229(4711):393-5 PMID: 3839317
  23. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  24. A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor.
    Science. 1987 Nov 6;238(4828):797-9 PMID: 3672127
  25. Induction of transiently expressed genes in PC-12 pheochromocytoma cells.
    Oncogene. 1987;1(3):257-62 PMID: 3502572
  26. A simple subtractive hybridization technique employing photoactivatable biotin and phenol extraction.
    Nucleic Acids Res. 1988 Nov 25;16(22):10937 PMID: 2462719
  27. Nerve growth factor regulates gene expression by several distinct mechanisms.
    Mol Cell Biol. 1989 Jan;9(1):135-43 PMID: 2538715
  28. Early gene regulation by nerve growth factor in PC12 cells: induction of an interferon-related gene.
    Proc Natl Acad Sci U S A. 1989 Mar;86(6):2088-92 PMID: 2467301
  29. Synaptotagmin II. A novel differentially distributed form of synaptotagmin.
    J Biol Chem. 1991 Jul 25;266(21):13548-52 PMID: 1856191
  30. Primary response genes induced by growth factors and tumor promoters.
    Annu Rev Biochem. 1991;60:281-319 PMID: 1883198
  31. Stimulus-transcription coupling in the nervous system: involvement of the inducible proto-oncogenes fos and jun.
    Annu Rev Neurosci. 1991;14:421-51 PMID: 1903243
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.6.2164
Published
1995-03-14
Pages
2164-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC42444
Subset
IM
Grants
NIA NIH HHS · AG00093 · United States
NINDS NIH HHS · NS28660 · United States
Databases
GENBANK
L38247
External Links
PubMed source DOI: 10.1073/pnas.92.6.2164 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2