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PMID: 7906416 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Analysis of the T-cell receptor beta-chain variable-region (V beta) repertoire in monozygotic twins discordant for human immunodeficiency virus: evidence for perturbations of specific V beta segments in CD4+ T cells of the virus-positive twins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 4 ·1994-02-15 ·Pages 1529-33

Rebai N, Pantaleo G, Demarest JF, Ciurli C, Soudeyns H, Adelsberger JW, Vaccarezza M, Walker RE, Sekaly RP, Fauci AS

Abstract

We analyzed the T-cell receptor (TCR) V beta repertoire in human immunodeficiency virus type 1 (HIV-1)-infected individuals at different stages of disease. To circumvent the effect of HLA and other loci on the expressed TCR repertoire, we compared the TCR repertoire in nine pairs of monozygotic twins who were discordant for HIV infection. A semiquantitative polymerase chain reaction (PCR) assay and flow cytometry enabled us to show distinct differences in the V beta repertoire in the HIV-positive twin compared with the HIV-negative twin. By combining PCR and cytofluorometry, these differences were restricted to a specific set of TCR V beta segments, with members of the V beta 13 family perturbed in six out of seven cases and those of the V beta 21 family perturbed in four out of seven cases studied. Most of the other V beta families remained unchanged. Our results provide direct evidence for a skewed TCR repertoire in HIV infection.

MeSH Terms
Antibodies, Monoclonal CD4-Positive T-Lymphocytes/immunology CD8 Antigens/immunology Flow Cytometry Genetic Variation HIV Infections/immunology HIV-1/immunology Humans Leukapheresis Polymerase Chain Reaction Receptors, Antigen, T-Cell, alpha-beta/genetics Superantigens/immunology T-Lymphocyte Subsets/immunology Twins, Monozygotic
Chemicals
Antibodies, Monoclonal CD8 Antigens Receptors, Antigen, T-Cell, alpha-beta Superantigens
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Rebai N
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
Pantaleo G
Demarest J F
Ciurli C
Soudeyns H
Adelsberger J W
Vaccarezza M
Walker R E
Sekaly R P
Fauci A S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.4.1529
Published
1994-02-15
Pages
1529-33
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43193
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.91.4.1529 PMC Full Text View journal details
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