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PMID: 1656442 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A method for production of antibodies to human T-cell receptor beta-chain variable regions.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 19 ·1991-10-01 ·Pages 8357-61

Choi YW, Kotzin B, Lafferty J, White J, Pigeon M, Kubo R, Kappler J, Marrack P

Abstract

Mouse T-cell hybridomas bearing human V beta elements were produced by transfection of human/mouse hybrid T-cell receptor beta-chain genes into a mouse T-cell hybridoma lacking an endogenous beta-chain gene. These hybridomas were entirely mouse in origin except for the human V beta region. These cells were used to immunize mice against human V beta elements. Mouse monoclonal antibodies have thus been generated against human V beta 13.1 and -13.2. We expect that the method outlined in this paper will be useful in the production of monoclonal antibodies specific for other human V beta or V alpha elements.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antibody Specificity Base Sequence Gene Rearrangement, beta-Chain T-Cell Antigen Receptor Humans Hybridomas Mice Molecular Sequence Data Multigene Family Oligonucleotides/chemistry Polymerase Chain Reaction Receptors, Antigen, T-Cell, alpha-beta/genetics,immunology Recombinant Fusion Proteins/immunology T-Lymphocytes/immunology Transfection
Chemicals
Antibodies, Monoclonal Oligonucleotides Receptors, Antigen, T-Cell, alpha-beta Recombinant Fusion Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Choi Y W
Howard Hughes Medical Institute, Denver, CO.
Kotzin B
Lafferty J
White J
Pigeon M
Kubo R
Kappler J
Marrack P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.19.8357
Published
1991-10-01
Pages
8357-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52507
Subset
IM
Grants
NIAID NIH HHS · AI17134 · United States
NIAID NIH HHS · AI18785 · United States
NIAID NIH HHS · AI22295 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.19.8357 PMC Full Text View journal details
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