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PMID: 7935457 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hierarchies of RNA-processing signals in a trypanosome surface antigen mRNA precursor.

Molecular and cellular biology ·Vol. 14 ·No. 11 ·1994-11-00 ·Pages 7428-35

Hug M, Hotz HR, Hartmann C, Clayton C

Abstract

Nearly all trypanosome mRNAs are synthesized as polycistronic precursors, from which mature mRNAs are excised by trans splicing and polyadenylation. Polyadenylation of a procyclic acidic repetitive protein (PARP, or procyclin) transcript was studied by transient transfection of constructs bearing a chloramphenicol acetyltransferase gene linked to the PARP intergenic region. Polyadenylation usually occurred at A residues, about 100 bases upstream of a trans-splicing acceptor signal. The wild-type polyadenylation site has a cryptic trans-splicing signal about 100 bp downstream: deletion or inversion of this signal results in polyadenylation at multiple sites, upstream of other cryptic trans-splicing signals. The PARP mRNA precursor appears to contain a hierarchy of possible processing signals, the function of cryptic ones being revealed only when the dominant ones are deleted or moved. Correct polyadenylation can be restored by addition of trans-splicing signals from other loci. The results indicate that polyadenylation is coupled to downstream trans splicing but that the products of the trans-splicing reaction are not necessarily functional mRNAs.

MeSH Terms
Animals Antigens, Protozoan/genetics Base Sequence DNA Primers/genetics DNA, Protozoan/genetics Molecular Sequence Data Poly A/genetics,metabolism Polymerase Chain Reaction RNA Precursors/genetics,metabolism RNA Processing, Post-Transcriptional RNA Splicing RNA, Protozoan/genetics,metabolism Trypanosoma brucei brucei/genetics,immunology,metabolism
Chemicals
Antigens, Protozoan DNA Primers DNA, Protozoan RNA Precursors RNA, Protozoan Poly A
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hug M
Zentrum für Molekulare Biologie, Heidelberg, Federal Republic of Germany.
Hotz H R
Hartmann C
Clayton C
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38 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.11.7428-7435.1994
Published
1994-11-00
Pages
7428-35
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359278
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.11.7428-7435.1994 PMC Full Text View journal details
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