Home LiteratureArticle Details
PMID: 8190625 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Control of polyadenylation and alternative splicing of transcripts from adjacent genes in a procyclin expression site: a dual role for polypyrimidine tracts in trypanosomes?

Nucleic acids research ·Vol. 22 ·No. 8 ·1994-04-25 ·Pages 1359-64

Vassella E, Braun R, Roditi I

Abstract

The procyclin-associated genes (PAGs) of Trypanosoma brucei are located downstream of tandemly repeated procyclin genes and belong to the same alpha-amanitin-resistant polycistronic transcription units. In procyclic form trypanosomes the PAG 1 pre-mRNA is alternatively spliced to give rise to three transcripts of 2.7 kb, 1.8 kb and 1.3 kb. The two larger transcripts contain additional short open reading frames (ORFs) upstream of the major ORF. Trans-splicing to generate these transcripts occurs downstream of three different polypyrimidine tracts. A minor population of procyclin mRNAs is also generated by alternative splicing at a polypyrimidine tract that begins 524 bp upstream of the major splice acceptor site of the procyclin beta-gene. The same polypyrimidine tract is also required for accurate polyadenylation of mRNAs from the upstream procyclin alpha-gene (1). Alternatively polyadenylated forms of PAG 1 mRNAs can also be detected. All polyadenylation sites are found at a similar distance upstream of splice-acceptor sites, in each case with a polypyrimidine tract between them. Our results point to a dual role for polypyrimidine tracts in the maturation of trypanosome mRNAs.

MeSH Terms
Alternative Splicing Animals Base Sequence DNA, Protozoan/genetics,metabolism Membrane Glycoproteins Molecular Sequence Data Open Reading Frames Poly A/metabolism Polymerase Chain Reaction Protozoan Proteins Pyrimidines/metabolism RNA, Messenger/metabolism Restriction Mapping Transcription, Genetic Trypanosoma brucei brucei/genetics Variant Surface Glycoproteins, Trypanosoma/biosynthesis,genetics
Chemicals
DNA, Protozoan Membrane Glycoproteins Protozoan Proteins Pyrimidines RNA, Messenger Variant Surface Glycoproteins, Trypanosoma procyclic acidic repetitive protein, Trypanosoma Poly A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vassella E
Institut für Allgemeine Mikrobiologie, Universität Bern, Switzerland.
Braun R
Roditi I
References (35)
35 references, click to expand
  1. Cultivation of Trypanosoma brucei sspp. in semi-defined and defined media.
    Parasitology. 1973 Dec;67(3):315-31 PMID: 4761771
  2. A conserved stem-loop structure in the 3' untranslated region of procyclin mRNAs regulates expression in Trypanosoma brucei.
    Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):370-4 PMID: 8278396
  3. Analysis of antigenic types appearing in first relapse populations of clones of Trypanosoma brucei.
    Parasitology. 1981 Feb;82(1):63-80 PMID: 6163126
  4. analysis of genomic rearrangements associated with two variable antigen genes of Trypanosoma brucei.
    Nucleic Acids Res. 1982 Feb 11;10(3):803-19 PMID: 6278436
  5. Alpha-amanitin-insensitive transcription of variant surface glycoprotein genes provides further evidence for discontinuous transcription in trypanosomes.
    Nucleic Acids Res. 1984 Dec 21;12(24):9457-72 PMID: 6514580
  6. Coordinate transcription of variant surface glycoprotein genes and an expression site associated gene family in Trypanosoma brucei.
    Cell. 1985 Aug;42(1):173-82 PMID: 2861910
  7. Multiple upstream AUG codons mediate translational control of GCN4.
    Cell. 1986 Apr 25;45(2):201-7 PMID: 3516411
  8. Discontinuous transcription and antigenic variation in trypanosomes.
    Annu Rev Biochem. 1986;55:701-32 PMID: 2874767
  9. Expression of a polypeptide containing a dipeptide repeat is confined to the insect stage of Trypanosoma brucei.
    Nature. 1987 Jan 15-21;325(6101):272-4 PMID: 3808022
  10. Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei.
    Mol Cell Biol. 1988 Jun;8(6):2367-78 PMID: 3405209
  11. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8998-9002 PMID: 2461560
  12. Procyclin: an unusual immunodominant glycoprotein surface antigen from the procyclic stage of African trypanosomes.
    Mol Biochem Parasitol. 1988 Dec;31(3):203-16 PMID: 2464763
  13. Procyclin gene expression and loss of the variant surface glycoprotein during differentiation of Trypanosoma brucei.
    J Cell Biol. 1989 Feb;108(2):737-46 PMID: 2645304
  14. Transcripts from the co-transposed segment of variant surface glycoprotein genes are in Trypanosoma brucei polyribosomes.
    Mol Biochem Parasitol. 1989 Jan 15;32(2-3):169-78 PMID: 2927445
  15. Control of antigen gene expression in African trypanosomes.
    Annu Rev Genet. 1988;22:107-26 PMID: 3071245
  16. The genes and transcripts of an antigen gene expression site from T. brucei.
    Cell. 1989 Jun 2;57(5):835-45 PMID: 2720787
  17. Variation of tandem repeats in the developmentally regulated procyclic acidic repetitive proteins of Trypanosoma brucei.
    Mol Cell Biol. 1989 Mar;9(3):1332-5 PMID: 2725502
  18. Trypanosoma brucei: posttranscriptional control of the variable surface glycoprotein gene expression site.
    Mol Cell Biol. 1989 Sep;9(9):4018-21 PMID: 2779574
  19. Trypanosoma brucei: enrichment by UV of intergenic transcripts from the variable surface glycoprotein gene expression site.
    Mol Cell Biol. 1989 Sep;9(9):4022-5 PMID: 2779575
  20. Duplication and transcription of procyclin genes in Trypanosoma brucei.
    Nucleic Acids Res. 1989 Nov 11;17(21):8727-39 PMID: 2573878
  21. Cellular and genetic aspects of antigenic variation in trypanosomes.
    Annu Rev Immunol. 1990;8:83-110 PMID: 2188680
  22. Procyclic acidic repetitive protein (PARP) genes located in an unusually small alpha-amanitin-resistant transcription unit: PARP promoter activity assayed by transient DNA transfection of Trypanosoma brucei.
    Mol Cell Biol. 1990 Jul;10(7):3492-504 PMID: 1694012
  23. Trans splicing of nuclear pre-mRNAs.
    Cell. 1990 Jun 29;61(7):1157-60 PMID: 2142018
  24. Trypanosoma brucei: constitutive activity of the VSG and procyclin gene promoters.
    EMBO J. 1990 Oct;9(10):3145-51 PMID: 1698609
  25. A gene from the VSG expression site of Trypanosoma brucei encodes a protein with both leucine-rich repeats and a putative zinc finger.
    Nucleic Acids Res. 1990 Dec 25;18(24):7299-303 PMID: 2259625
  26. Mechanisms of alternative pre-mRNA splicing.
    Science. 1991 Jan 4;251(4989):33-4 PMID: 1824726
  27. A similar gene is shared by both the variant surface glycoprotein and procyclin gene transcription units of Trypanosoma brucei.
    Mol Cell Biol. 1991 Mar;11(3):1473-9 PMID: 1996104
  28. An expression-site-associated gene family of trypanosomes is expressed in vivo and shows homology to a variant surface glycoprotein gene.
    Mol Biochem Parasitol. 1990 Nov;43(1):1-16 PMID: 2290439
  29. A family of genes related to a new expression site-associated gene in Trypanosoma equiperdum.
    Mol Cell Biol. 1991 Apr;11(4):2180-8 PMID: 1672441
  30. Requirement of a polypyrimidine tract for trans-splicing in trypanosomes: discriminating the PARP promoter from the immediately adjacent 3' splice acceptor site.
    EMBO J. 1991 Dec;10(12):3877-85 PMID: 1935907
  31. Role of an upstream open reading frame in the translation of polycistronic mRNAs in plant cells.
    Nucleic Acids Res. 1992 Aug 11;20(15):3851-7 PMID: 1508670
  32. Alternative mRNA splicing.
    Annu Rev Cell Biol. 1992;8:133-55 PMID: 1335742
  33. Operons in C. elegans: polycistronic mRNA precursors are processed by trans-splicing of SL2 to downstream coding regions.
    Cell. 1993 May 7;73(3):521-32 PMID: 8098272
  34. Coupling of poly(A) site selection and trans-splicing in Leishmania.
    Genes Dev. 1993 Jun;7(6):996-1007 PMID: 8504937
  35. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.8.1359
Published
1994-04-25
Pages
1359-64
Language
English
Region
England
NLM ID
0411011
PMCID
PMC307990
Subset
IM
Databases
GENBANK
S70405, S70406, S70407, S70408
External Links
PubMed source DOI: 10.1093/nar/22.8.1359 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2