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PMID: 8417363 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Temporal order of RNA-processing reactions in trypanosomes: rapid trans splicing precedes polyadenylation of newly synthesized tubulin transcripts.

Molecular and cellular biology ·Vol. 13 ·No. 1 ·1993-01-00 ·Pages 720-5

Ullu E, Matthews KR, Tschudi C

Abstract

Many trypanosome genes are expressed as part of large polycistronic transcription units. This finding suggests that regulation of mRNA biogenesis may emphasize RNA-processing reactions more so than in other organisms. This study was undertaken to understand the temporal order of two RNA-processing reactions, trans splicing and polyadenylation, in the maturation of trypanosome mRNAs in vivo. Kinetic studies revealed rapid trans splicing of alpha-tubulin, beta-tubulin, and actin pre-mRNAs within 1 to 2 min after synthesis of the 3' splice site. Furthermore, following blockage of pre-mRNA synthesis, newly synthesized spliced leader RNA cannot be used for trans splicing, suggesting that trypanosomes do not accumulate substantial amounts of pre-mRNA which can provide splice acceptor sites. Thus, trans splicing is cotranscriptional. In addition, we show that trans splicing precedes polyadenylation in the processing of trypanosome tubulin pre-mRNAs.

MeSH Terms
Animals Gene Expression Regulation Genes, Protozoan Nucleic Acid Precursors/metabolism Poly A/metabolism RNA Splicing RNA, Messenger/genetics RNA, Protozoan/genetics Time Factors Trypanosoma brucei brucei/genetics Tubulin/genetics
Chemicals
Nucleic Acid Precursors RNA, Messenger RNA, Protozoan Tubulin Poly A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ullu E
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510-8056.
Matthews K R
Tschudi C
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34 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.1.720-725.1993
Published
1993-01-00
Pages
720-5
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358950
Subset
IM
Grants
NIAID NIH HHS · AI-28798 · United States
External Links
PubMed source DOI: 10.1128/mcb.13.1.720-725.1993 PMC Full Text View journal details
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