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PMID: 7937089 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Formamidopyrimidine DNA glycosylase in the yeast Saccharomyces cerevisiae.

Nucleic acids research ·Vol. 22 ·No. 18 ·1994-09-11 ·Pages 3760-4

de Oliveira R, van der Kemp PA, Thomas D, Geiger A, Nehls P, Boiteux S

Abstract

A DNA glycosylase that excises, 2,6-diamino-4-hydroxy-5N-methylformamidopyrimidine (Fapy) from double stranded DNA has been purified 28,570-fold from the yeast Saccharomyces cerevisiae. Gel filtration chromatography shows that yeast Fapy DNA glycosylase has a molecular weight of about 40 kDa. The Fapy DNA glycosylase is active in the presence of EDTA, but is completely inhibited by 0.2 M KCl. Yeast Fapy DNA glycosylase does not excise N7-methylguanine, N3-methyladenine or uracil. A repair enzyme for 7,8-dihydro-8-oxoguanine (8-OxoG) co-purifies with the Fapy DNA glycosylase. This repair activity causes strand cleavage at the site of 8-OxoG in DNA duplexes. The highest rate of incision of the 8-OxoG-containing strand was observed for duplexes where 8-OxoG was opposite guanine. The mode of incision at 8-OxoG was not established yet. The results however suggest that the Fapy- and 8-OxoG-repair activities are associated with a single protein.

MeSH Terms
Base Sequence DNA Repair/physiology DNA-Formamidopyrimidine Glycosylase Guanine/analogs & derivatives,metabolism Molecular Sequence Data Molecular Weight N-Glycosyl Hydrolases/chemistry,isolation & purification,metabolism Polydeoxyribonucleotides/chemical synthesis,metabolism Saccharomyces cerevisiae/enzymology
Chemicals
Polydeoxyribonucleotides 8-hydroxyguanine Guanine N-Glycosyl Hydrolases DNA-Formamidopyrimidine Glycosylase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
de Oliveira R
Groupe Réparation des lésions radio et chimioinduites, URA147 CNRS, Institut Gustave Roussy, Villejuif, France.
van der Kemp P A
Thomas D
Geiger A
Nehls P
Boiteux S
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.18.3760
Published
1994-09-11
Pages
3760-4
Language
English
Region
England
NLM ID
0411011
PMCID
PMC308359
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/22.18.3760 PMC Full Text View journal details
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