The kinetics of bisulphite modification of reactive residues in E. coli tRNA2Phe.

Nucleic acids research ·Vol. 3 ·No. 12 ·1976-12-00 ·Pages 3383-96

Lowdon M, Goddard JP

Abstract

E coli tRNA2Phe was modified at 25 degrees C with 3M sodium bisulphite, pH6.0, for periods of up to 48 hours, Three cytadinine residues, at position 17, 74 and 75 from the 5' end were each deaminated to uridine. The 2-methylthio-N6-isopentenyl adenosine at position 37 formed a 1:1 bi-sulphite addition product which was stable to alkaii. No other residues were permanently modified. The rate of modification of each residue was first order with respect to remaining unmodified nucleotide, the time of half reaction, t1/2, being different for each residue. C17 reaction reacted at twice the rate of cytidine in PolyC, indicating that it occupied a very exposed position in the tRNA.

MeSH Terms

Base Sequence Chemical Phenomena Chemistry Escherichia coli/metabolism Kinetics Nucleic Acid Conformation Phenylalanine RNA, Bacterial RNA, Transfer Structure-Activity Relationship Sulfites/pharmacology

Chemicals

RNA, Bacterial Sulfites Phenylalanine RNA, Transfer

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Lowdon M

Goddard J P

References (28)

28 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/3.12.3383

Published

1976-12-00

Pages

3383-96

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343183

Subset

External Links

PubMed source DOI: 10.1093/nar/3.12.3383 PMC Full Text View journal details

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The kinetics of bisulphite modification of reactive residues in E. coli tRNA2Phe.

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