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PMID: 796664 Published · ppublish English Journal Article

A map of the restriction targets in yeast 2 micron plasmid DNA cloned on bacteriophage lambda.

Molecular & general genetics : MGG ·Vol. 148 ·No. 3 ·1976-11-17 ·Pages 287-94

Beggs JD, Guerineau M, Atkins JF

Abstract

The 2 micron circular DNA from S. cerevisiae has been cloned on bacteriophage lambda. The two forms of circular DNA which exist in equilibrium due to recombination between inverted repeat sequences were separated as stable clones, and a map of targets for restriction endonucleases EcoRI, HindIII and HpaI was constructed. The circular DNAs isolated from a particular oligomycin resistant strain and its parent oligomycin snesitive strain were compared by restriction endonuclease analysis, and no difference was detected. The potential uses of cloned 2 micron DNA in determining the possible biological role of these plasmids are considered.

MeSH Terms
Coliphages DNA Restriction Enzymes DNA, Circular DNA, Recombinant Extrachromosomal Inheritance Genes Genetic Linkage Molecular Conformation Plasmids Saccharomyces cerevisiae
Chemicals
DNA, Circular DNA, Recombinant DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Beggs J D
Guerineau M
Atkins J F
References (32)
32 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00332903
Published
1976-11-17
Pages
287-94
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00332903
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