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PMID: 7980428 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of transferrin receptor recycling by protein phosphorylation.

The Biochemical journal ·Vol. 303 ( Pt 2) ·1994-10-15 ·Pages 647-55

Beauchamp JR, Woodman PG

Abstract

The effect of the protein phosphatase inhibitor okadaic acid on transferrin receptor internalization and recycling was examined in HeLa and K562 cells. Okadaic acid inhibited receptor uptake by more than 85% in both cell lines, whereas it affected transferrin recycling to differing degrees: recycling in HeLa cells was inhibited by greater than 90%, compared with only 65% in K562 cells. Okadaic acid also caused a marked redistribution of receptors in each cell line, which was accounted for by the difference in the extent to which transferrin uptake and recycling were inhibited. These effects were most likely mediated by a protein kinase, as they were delayed by 10-15 min and could be suppressed by prior incubation with certain protein kinase inhibitors. In addition, it was found that specific kinase inhibitors affected basal rates of transferrin uptake and recycling, although the extent of these effects differed between cell lines. Together, these results suggest that a complex pattern of protein phosphorylation influences the flux of the endocytic pathway in interphase cells.

MeSH Terms
Adenosine Triphosphate/metabolism Alkaloids/pharmacology Binding Sites Cell Line Cell Survival/drug effects DNA/metabolism Deferoxamine/pharmacology Endocytosis/drug effects Ethers, Cyclic/metabolism,pharmacology HeLa Cells Humans Isotope Labeling Okadaic Acid Phosphoprotein Phosphatases/antagonists & inhibitors Phosphorylation Protein Kinase Inhibitors Protein Kinases/metabolism Receptors, Transferrin/drug effects,metabolism Staurosporine Transferrin/metabolism
Chemicals
Alkaloids Ethers, Cyclic Protein Kinase Inhibitors Receptors, Transferrin Transferrin Okadaic Acid Adenosine Triphosphate DNA Protein Kinases Phosphoprotein Phosphatases Staurosporine Deferoxamine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Beauchamp J R
Division of Biochemistry, School of Biological Sciences, University of Manchester Medical School, U.K.
Woodman P G
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj3030647
Published
1994-10-15
Pages
647-55
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1137376
Subset
IM
External Links
PubMed source DOI: 10.1042/bj3030647 PMC Full Text View journal details
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