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PMID: 7985999 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular and genetic analysis of the Bacteroides uniformis cephalosporinase gene, cblA, encoding the species-specific beta-lactamase.

Antimicrobial agents and chemotherapy ·Vol. 38 ·No. 8 ·1994-08-00 ·Pages 1711-5

Smith CJ, Bennett TK, Parker AC

Abstract

The gene, cblA, encoding the species-specific, clavulanate-susceptible, endogenous cephalosporinase was cloned from Bacteroides uniformis WAL-7088. The nucleotide sequence was determined, and the cblA structural gene was found to be 891 nucleotides, with a 48% G+C composition, which is similar to that of the B. uniformis genome. The cblA open reading frame encoded an Ambler class A beta-lactamase polypeptide precursor of 296 amino acid residues with a predicted molecular weight of 33,450. A beta-lactamase-deficient B. uniformis mutant with increased beta-lactam susceptibility was constructed by insertional inactivation of the chromosomal gene. This mutant was complemented by plasmids bearing the cblA gene, and the resulting strains were resistant to cephaloridine and had a beta-lactamase that comigrated with the parental beta-lactamase on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (30,500 Da) and in isoelectric focusing gels (pI 4.6), confirming a role for this beta-lactamase in resistance.

Related Genes
cblA
MeSH Terms
Amino Acid Sequence Bacteroides/enzymology,genetics Base Sequence Cephalosporinase/analysis,chemistry,genetics Cloning, Molecular Escherichia coli/genetics Genes, Bacterial Molecular Sequence Data Species Specificity
Chemicals
Cephalosporinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Smith C J
Department of Microbiology and Immunology, East Carolina University, North Carolina 27858.
Bennett T K
Parker A C
References (30)
30 references, click to expand
  1. Characterization of beta-lactamases.
    Antimicrob Agents Chemother. 1989 Mar;33(3):259-63 PMID: 2658779
  2. Characterization of cephalosporinases from Bacteroides fragilis, Bacteroides thetaiotaomicron and Bacteroides vulgatus.
    J Antibiot (Tokyo). 1983 Jan;36(1):76-85 PMID: 6332105
  3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  4. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  5. Cloning and characterization of the endogenous cephalosporinase gene, cepA, from Bacteroides fragilis reveals a new subgroup of Ambler class A beta-lactamases.
    Antimicrob Agents Chemother. 1993 Nov;37(11):2391-400 PMID: 8285623
  6. Identification of a circular intermediate in the transfer and transposition of Tn4555, a mobilizable transposon from Bacteroides spp.
    J Bacteriol. 1993 May;175(9):2682-91 PMID: 8386723
  7. Escherichia coli chromosomal mutations that permit direct cloning of the Bacteroides fragilis metallo-beta-lactamase gene, ccrA.
    Mol Microbiol. 1991 May;5(5):1211-9 PMID: 1956298
  8. Transferable lincosamide-macrolide resistance in Bacteroides.
    Plasmid. 1979 Apr;2(2):261-8 PMID: 451051
  9. The beta-lactamases of Bacteroides species.
    J Antimicrob Chemother. 1981 Feb;7(2):137-46 PMID: 6971286
  10. A Bacteroides tetracycline resistance gene represents a new class of ribosome protection tetracycline resistance.
    Antimicrob Agents Chemother. 1992 May;36(5):1005-12 PMID: 1339256
  11. Genetic and biochemical analysis of a novel Ambler class A beta-lactamase responsible for cefoxitin resistance in Bacteroides species.
    Antimicrob Agents Chemother. 1993 May;37(5):1028-36 PMID: 8517690
  12. The beta-lactamases of genus Bacteroides.
    J Antibiot (Tokyo). 1983 Apr;36(4):423-8 PMID: 6602121
  13. Characterization of three different beta-lactamases from the Bacteroides fragilis group.
    Antimicrob Agents Chemother. 1980 Aug;18(2):220-5 PMID: 6969573
  14. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.
    Antimicrob Agents Chemother. 1972 Apr;1(4):283-8 PMID: 4208895
  15. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  16. Molecular analysis and nucleotide sequence of the envCD operon of Escherichia coli.
    Mol Gen Genet. 1991 Nov;230(1-2):230-40 PMID: 1720861
  17. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  18. Classification of beta-lactamases: groups 2c, 2d, 2e, 3, and 4.
    Antimicrob Agents Chemother. 1989 Mar;33(3):271-6 PMID: 2658781
  19. Sequencing the gene for an imipenem-cefoxitin-hydrolyzing enzyme (CfiA) from Bacteroides fragilis TAL2480 reveals strong similarity between CfiA and Bacillus cereus beta-lactamase II.
    J Bacteriol. 1990 May;172(5):2584-93 PMID: 2110145
  20. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  21. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  22. Heterologous gene expression in Bacteroides fragilis.
    Plasmid. 1992 Mar;27(2):141-54 PMID: 1615064
  23. Sensitivity of four species of bacteroides to antibiotics.
    Br Med J. 1955 Dec 24;2(4955):1529-31 PMID: 13269908
  24. Plasmid transfer from Escherichia coli to Bacteroides fragilis: differential expression of antibiotic resistance phenotypes.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7203-6 PMID: 6095273
  25. Structure and regulation of the AMP nucleosidase gene (amn) from Escherichia coli.
    Biochemistry. 1989 Oct 31;28(22):8726-33 PMID: 2690948
  26. Resistance to beta-lactam antibiotics in anaerobic bacteria.
    Rev Infect Dis. 1990 Jan-Feb;12 Suppl 2:S231-4 PMID: 2406875
  27. Evidence that the clindamycin-erythromycin resistance gene of Bacteroides plasmid pBF4 is on a transposable element.
    J Bacteriol. 1985 May;162(2):626-32 PMID: 2985540
  28. Activation of a cryptic streptomycin-resistance gene in the Bacteroides erm transposon, Tn4551.
    Mol Microbiol. 1992 Aug;6(16):2287-97 PMID: 1328814
  29. Alterations to the penicillin-binding proteins in the Bacteroides fragilis group: a mechanism for non-beta-lactamase mediated cefoxitin resistance.
    J Antimicrob Chemother. 1990 Jul;26(1):7-20 PMID: 2211448
  30. Characterization of Bacteroides ovatus plasmid pBI136 and structure of its clindamycin resistance region.
    J Bacteriol. 1985 Mar;161(3):1069-73 PMID: 2982783
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.38.8.1711
Published
1994-08-00
Pages
1711-5
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC284626
Subset
IM
Grants
NIAID NIH HHS · AI-28884 · United States
Databases
GENBANK
L08472
External Links
PubMed source DOI: 10.1128/AAC.38.8.1711 PMC Full Text View journal details
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