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PMID: 8036154 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

c-Myc binds to 5' flanking sequence motifs of the dihydrofolate reductase gene in cellular extracts: role in proliferation.

Nucleic acids research ·Vol. 22 ·No. 12 ·1994-06-25 ·Pages 2264-73

Mai S, Jalava A

Abstract

The dihydrofolate reductase is a key enzyme of the folate metabolism which supplies the cell with dTTPs for DNA synthesis. Using cellular extracts, we demonstrate the formation of c-Myc/Max heterodimers at the dihydrofolate reductase (DHFR) 5' flanking CANNTG (E-box) motifs. The presence of these complexes correlates with c-Myc levels and active cellular proliferation.

Related Genes
DHFR
MeSH Terms
Amino Acid Sequence Animals Base Sequence Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Basic-Leucine Zipper Transcription Factors Binding Sites Cell Division Cell Line Cells, Cultured Cricetinae DNA/metabolism DNA-Binding Proteins/metabolism Humans Molecular Sequence Data Proto-Oncogene Proteins c-myc/metabolism Tetrahydrofolate Dehydrogenase/genetics,metabolism Transcription Factors
Chemicals
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Basic-Leucine Zipper Transcription Factors DNA-Binding Proteins MAX protein, human Myc associated factor X Proto-Oncogene Proteins c-myc Transcription Factors DNA Tetrahydrofolate Dehydrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mai S
Basel Institute for Immunology, Switzerland.
Jalava A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.12.2264
Published
1994-06-25
Pages
2264-73
Language
English
Region
England
NLM ID
0411011
PMCID
PMC523683
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/22.12.2264 PMC Full Text View journal details
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