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PMID: 80399 Published · ppublish English Journal Article

Conjugation of lectins with fluorochromes: an approach to histochemical double labeling of carbohydrate components.

Histochemistry ·Vol. 56 ·No. 3-4 ·1978-07-12 ·Pages 265-73

Roth J, Binder M, Gerhard UJ

Abstract

Methodical investigations on the coupling of lectins (Con A, LcL, WGA, RcA) to tetramethylrhodamine isothiocyan ate (TRITC) are reported. 20-microgram of TRITC per mg of lectin were found to be the optimal amount of TRITC for the conjugation. With this fluorochrome: protein ratio conjugates were produced which resulted in a specific and brilliant fluorescence in tissue staining. The optimally conjugated lectins were separated on DEAE-Sephadex-A 50. Using two different lectins which were conjugated with TRITC or FITC, respectively, a double labeling of different lectin-binding sites in tissue sections was achieved.

MeSH Terms
Animals Binding Sites, Antibody Carbohydrates/analysis Colon/analysis Lectins Myocardium/analysis Rats Rhodamines Staining and Labeling/methods Xanthenes
Chemicals
Carbohydrates Lectins Rhodamines Xanthenes tetramethylrhodamine isothiocyanate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Roth J
Binder M
Gerhard U J
References (37)
37 references, click to expand
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Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00495988
Published
1978-07-12
Pages
265-73
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00495988
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