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PMID: 8057484 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of Epstein-Barr virus (EBV)-negative cell clones from the EBV-positive Burkitt's lymphoma (BL) line Akata: malignant phenotypes of BL cells are dependent on EBV.

Journal of virology ·Vol. 68 ·No. 9 ·1994-09-00 ·Pages 6069-73

Shimizu N, Tanabe-Tochikura A, Kuroiwa Y, Takada K

Abstract

During cultivation of the Epstein-Barr virus (EBV)-positive Burkitt's lymphoma (BL) line Akata, it was noted that EBV DNA is lost from some of the cells. Isolation of EBV-positive and EBV-negative clones with the same origin made it possible to examine the effects of EBV in BL cells. The results indicate that malignant phenotypes of BL, such as growth in low serum, anchorage-independent growth in soft agar, and tumorigenicity in nude mice, are dependent on the presence of EBV genomes and underline the oncogenic function of EBV in human cancer.

MeSH Terms
Animals Antigens, Viral/analysis Base Sequence Burkitt Lymphoma/microbiology,pathology Cell Division DNA Primers/chemistry DNA-Binding Proteins/analysis Epstein-Barr Virus Nuclear Antigens Herpesvirus 4, Human/pathogenicity Humans Mice Mice, Nude Molecular Sequence Data Tumor Cells, Cultured/microbiology,pathology
Chemicals
Antigens, Viral DNA Primers DNA-Binding Proteins Epstein-Barr Virus Nuclear Antigens
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shimizu N
Department of Virology and Parasitology, Yamaguchi University School of Medicine, Japan.
Tanabe-Tochikura A
Kuroiwa Y
Takada K
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.9.6069-6073.1994
Published
1994-09-00
Pages
6069-73
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC237015
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.68.9.6069-6073.1994 PMC Full Text View journal details
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