Home LiteratureArticle Details
PMID: 2462063 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Synchronous and sequential activation of latently infected Epstein-Barr virus genomes.

Journal of virology ·Vol. 63 ·No. 1 ·1989-01-00 ·Pages 445-9

Takada K, Ono Y

Abstract

A lymphoid cell system was established that can induce the prompt and synchronous activation of latent Epstein-Barr virus (EBV) genomes and thus allows the identification of viral genes that are activated sequentially depending on their functions. With this system, we proved that disruption of EBV latency is initiated by activation of four EBV genes and that protein synthesis is not required prior to activation of latent EBV. The system should be an in vitro model for studying the mechanism of herpesvirus latency.

MeSH Terms
Blotting, Northern Blotting, Southern Burkitt Lymphoma Cytoplasm/analysis DNA Probes DNA, Viral/analysis Gene Expression Regulation Genes, Viral Herpesvirus 4, Human/genetics Humans RNA/analysis Restriction Mapping Tumor Cells, Cultured
Chemicals
DNA Probes DNA, Viral RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Takada K
Department of Microbiology, Nihon University School of Medicine, Tokyo, Japan.
Ono Y
References (35)
35 references, click to expand
  1. Promiscuous trans activation of gene expression by an Epstein-Barr virus-encoded early nuclear protein.
    J Virol. 1986 Oct;60(1):140-8 PMID: 3018281
  2. Characterization of two related Epstein-Barr virus-encoded membrane proteins that are differentially expressed in Burkitt lymphoma and in vitro-transformed cell lines.
    Proc Natl Acad Sci U S A. 1986 Aug;83(15):5703-7 PMID: 3016715
  3. Restricted expression of EBV latent genes and T-lymphocyte-detected membrane antigen in Burkitt's lymphoma cells.
    EMBO J. 1986 Oct;5(10):2599-607 PMID: 3023050
  4. trans activation of an Epstein-Barr viral transcriptional enhancer by the Epstein-Barr viral nuclear antigen 1.
    Mol Cell Biol. 1986 Nov;6(11):3838-46 PMID: 3025615
  5. Both Epstein-Barr virus (EBV)-encoded trans-acting factors, EB1 and EB2, are required to activate transcription from an EBV early promoter.
    EMBO J. 1986 Dec 1;5(12):3243-9 PMID: 3028777
  6. Regulation of inducible and tissue-specific gene expression.
    Science. 1987 Jun 5;236(4806):1237-45 PMID: 3296191
  7. Epstein-Barr virus gene expression in P3HR1-superinfected Raji cells.
    J Virol. 1987 Oct;61(10):3120-32 PMID: 3041034
  8. Both the rightward and the leftward open reading frames within the BamHI M DNA fragment of Epstein-Barr virus act as trans-activators of gene expression.
    J Virol. 1987 Oct;61(10):3310-3 PMID: 3041051
  9. Differences in B cell growth phenotype reflect novel patterns of Epstein-Barr virus latent gene expression in Burkitt's lymphoma cells.
    EMBO J. 1987 Sep;6(9):2743-51 PMID: 2824192
  10. TPA-inducible Epstein-Barr virus genes in Raji cells and their regulation.
    Virology. 1988 Feb;162(2):503-7 PMID: 2829433
  11. Immunofluorescence and herpes-type virus particles in the P3HR-1 Burkitt lymphoma cell line.
    J Virol. 1967 Oct;1(5):1045-51 PMID: 4912237
  12. Immunofluorescence in cells derived from Burkitt's lymphoma.
    J Bacteriol. 1966 Mar;91(3):1248-56 PMID: 4160230
  13. Latency comes of age for herpesviruses.
    Cell. 1988 Mar 25;52(6):787-9 PMID: 2832064
  14. Differential reactivity of human serums with early antigens induced by Epstein-Barr virus.
    Science. 1970 Jul 10;169(3941):188-90 PMID: 4316788
  15. Preparation, molecular weight, base composition, and secondary structure of giant nuclear ribonucleic acid.
    Biochemistry. 1973 Jun 5;12(12):2330-8 PMID: 4710584
  16. Proteins of Epstein-Barr virus. I. Analysis of the polypeptides of purified enveloped Epstein-Barr virus.
    J Virol. 1976 Mar;17(3):935-49 PMID: 176452
  17. Transforming activity of Epstein-Barr virus obtained by superinfection of Raji cells.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):2008-10 PMID: 205877
  18. Epstein-Barr virus (B95-8) DNA VII: molecular cloning and detailed mapping.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2999-3003 PMID: 6248879
  19. Epstein-Barr virus RNA. V. Viral RNA in a restringently infected, growth-transformed cell line.
    J Virol. 1980 Nov;36(2):506-18 PMID: 6253674
  20. Cloning and mapping of BamHi endonuclease fragments of DNA from the transforming B95-8 strain of Epstein-Barr virus.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3860-4 PMID: 6253994
  21. Epstein-Barr virus DNA. IX. Variation among viral DNAs from producer and nonproducer infected cells.
    J Virol. 1981 May;38(2):632-48 PMID: 6264134
  22. Characterization of the major Epstein-Barr virus-specific RNA in Burkitt lymphoma-derived cells.
    J Virol. 1982 Feb;41(2):376-89 PMID: 6281455
  23. Epstein-Barr virus RNA. VIII. Viral RNA in permissively infected B95-8 cells.
    J Virol. 1982 Jul;43(1):262-72 PMID: 6180174
  24. Stable expression in mouse cells of nuclear neoantigen after transfer of a 3.4-megadalton cloned fragment of Epstein-Barr virus DNA.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5688-92 PMID: 6291059
  25. Major EB virus-specific cytoplasmic transcripts in a cellular clone of the HR-1 Burkitt lymphoma line during latency and after induction of viral replicative cycle by phorbol esters.
    Virology. 1983 Mar;125(2):287-98 PMID: 6301144
  26. Two distant clusters of partially homologous small repeats of Epstein-Barr virus are transcribed upon induction of an abortive or lytic cycle of the virus.
    J Virol. 1983 Dec;48(3):731-43 PMID: 6313969
  27. Cross-linking of cell surface immunoglobulins induces Epstein-Barr virus in Burkitt lymphoma lines.
    Int J Cancer. 1984 Jan 15;33(1):27-32 PMID: 6319296
  28. Immortalization of monkey epithelial cells by specific fragments of Epstein-Barr virus DNA.
    Nature. 1984 May 3-9;309(5963):78-82 PMID: 6325929
  29. DNA sequence and expression of the B95-8 Epstein-Barr virus genome.
    Nature. 1984 Jul 19-25;310(5974):207-11 PMID: 6087149
  30. Identification of Epstein-Barr virus genes expressed during the early phase of virus replication and during lymphocyte immortalization.
    Virology. 1984 Nov;139(1):1-10 PMID: 6093376
  31. A membrane protein encoded by Epstein-Barr virus in latent growth-transforming infection.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7207-11 PMID: 6095274
  32. Stable replication of plasmids derived from Epstein-Barr virus in various mammalian cells.
    Nature. 1985 Feb 28-Mar 6;313(6005):812-5 PMID: 2983224
  33. A second nuclear protein is encoded by Epstein-Barr virus in latent infection.
    Science. 1985 Mar 8;227(4691):1238-40 PMID: 2983420
  34. Activation of expression of latent Epstein-Barr herpesvirus after gene transfer with a small cloned subfragment of heterogeneous viral DNA.
    Proc Natl Acad Sci U S A. 1985 Jun;82(12):4085-9 PMID: 2987963
  35. Identification and mapping of Epstein-Barr virus early antigens and demonstration of a viral gene activator that functions in trans.
    J Virol. 1986 Oct;60(1):149-56 PMID: 3018282
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.1.445-449.1989
Published
1989-01-00
Pages
445-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247705
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.63.1.445-449.1989 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2