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PMID: 8065315 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The PARP and rRNA promoters of Trypanosoma brucei are composed of dissimilar sequence elements that are functionally interchangeable.

Molecular and cellular biology ·Vol. 14 ·No. 9 ·1994-09-00 ·Pages 5804-11

Janz L, Clayton C

Abstract

The African trypanosomes express two major surface proteins, the variant surface glycoprotein (VSG) and the procyclic acidic repetitive protein (PARP). The RNA polymerase that transcribes the VSG and PARP genes shares many characteristics with RNA polymerase I. We show that although there is very little similarity in nucleotide sequence, the functional structure of a trypanosome rRNA promoter is almost identical to that of the PARP promoter. Further, domains from the PARP promoter can functionally substitute for the corresponding parts of the rRNA promoter, and vice versa.

MeSH Terms
Animals Base Sequence DNA Primers/chemistry Gene Expression Regulation Genes, Protozoan Membrane Glycoproteins Molecular Sequence Data Promoter Regions, Genetic Protozoan Proteins RNA, Ribosomal/genetics Structure-Activity Relationship Transcription, Genetic Trypanosoma brucei brucei/genetics Variant Surface Glycoproteins, Trypanosoma/genetics
Chemicals
DNA Primers Membrane Glycoproteins Protozoan Proteins RNA, Ribosomal Variant Surface Glycoproteins, Trypanosoma procyclic acidic repetitive protein, Trypanosoma
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Janz L
Zentrum für Molekulare Biologie, Heidelberg, Germany.
Clayton C
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43 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.9.5804-5811.1994
Published
1994-09-00
Pages
5804-11
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359106
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.9.5804-5811.1994 PMC Full Text View journal details
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