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PMID: 8070401 Published · ppublish English Comparative Study Journal Article

Tenm, a Drosophila gene related to tenascin, is a new pair-rule gene.

The EMBO journal ·Vol. 13 ·No. 16 ·1994-08-15 ·Pages 3728-40

Baumgartner S, Martin D, Hagios C, Chiquet-Ehrismann R

Abstract

We describe the molecular characterization of the Drosophila gene tenm, a large transcription unit spanning > 110 kb of DNA. tenm encodes a large extracellular protein of 2515 amino acids related to the extracellular matrix molecule tenascin. The Tenm protein is found in seven stripes during the blastoderm stage, and each stripe overlaps with the even-skipped stripes. tenm mutants show a phenotype resembling that of odd-paired (opa), a member of the pair-rule class of segmentation genes. Thus, Tenm is the first example of a pair-rule gene product acting from outside the cell. While the Tenm protein is under the control of fushi tarazu and even-skipped, but not of opa, at least two pair-rule genes, paired (prd) and sloppy paired (slp), and all segment-polarity genes analysed to date are under the control of tenm. Our data suggest that Tenm initiates a signal transduction cascade which acts, via or in concert with opa, on downstream targets such as prd, slp, gooseberry, engrailed and wingless, leading to an opa-like phenotype.

MeSH Terms
Amino Acid Sequence Animals Blastoderm/physiology Cloning, Molecular Drosophila/embryology,genetics Drosophila Proteins Extracellular Matrix Proteins/genetics,immunology,metabolism Genes, Insect/genetics Homeodomain Proteins In Situ Hybridization Models, Genetic Molecular Sequence Data Mutation Phenotype RNA, Messenger/analysis Recombinant Fusion Proteins/immunology Sequence Analysis, DNA Signal Transduction Tenascin Transcription Factors/metabolism Transcription, Genetic
Chemicals
Drosophila Proteins Extracellular Matrix Proteins Homeodomain Proteins Opa protein, Drosophila RNA, Messenger Recombinant Fusion Proteins Ten-m protein, Drosophila Tenascin Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Baumgartner S
Friedrich Miescher-Institut, Basel, Switzerland.
Martin D
Hagios C
Chiquet-Ehrismann R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-08-15
Pages
3728-40
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395283
Subset
IM
Databases
GENBANK
X73154
External Links
PubMed source PMC Full Text View journal details
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