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PMID: 8070662 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A Drosophila third chromosome Minute locus encodes a ribosomal protein.

Genetics ·Vol. 137 ·No. 2 ·1994-06-00 ·Pages 513-20

Andersson S, Saebøe-Larssen S, Lambertsson A, Merriam J, Jacobs-Lorena M

Abstract

Minutes (M) are a group of over 50 phenotypically similar Drosophila mutations widely believed to affect ribosomal protein genes. This report describes the characterization of the P element-induced M(3)95A(Plac92) mutation [allelic to M(3)95A]. This mutation can be reversed by the mobilization of the P element, demonstrating that the mutation is caused by insertion of this transposable element. The gene interrupted by insertion of the P element was cloned by use of inverse polymerase chain reaction. Nucleotide sequence analysis revealed a 70-75% identity to the human and rat ribosomal protein S3 genes, and to the Xenopus ribosomal protein S1a gene. At the amino acid level, the overall identity is approximately 78% for all three species. This is only the second time that a Minute has been demonstrated to encode a ribosomal protein.

Related Genes
rpS1&agr rpS3
MeSH Terms
Amino Acid Sequence Animals Base Sequence Chromosome Mapping Cloning, Molecular Crosses, Genetic Drosophila melanogaster/genetics Female Humans Male Molecular Sequence Data Mutation Phenotype Rats Restriction Mapping Ribosomal Proteins/biosynthesis,genetics Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Xenopus laevis
Chemicals
Ribosomal Proteins ribosomal protein S1 ribosomal protein S3
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Andersson S
Department of Genetics, University of Umeå, Sweden.
Saebøe-Larssen S
Lambertsson A
Merriam J
Jacobs-Lorena M
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/137.2.513
Published
1994-06-00
Pages
513-20
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1205974
Subset
IM
Databases
GENBANK
X72921
External Links
PubMed source DOI: 10.1093/genetics/137.2.513 PMC Full Text View journal details
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