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PMID: 8076605 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A functional interaction between Rev and yeast pre-mRNA is related to splicing complex formation.

The EMBO journal ·Vol. 13 ·No. 17 ·1994-09-01 ·Pages 4096-104

Stutz F, Rosbash M

Abstract

The HIV-1 Rev protein regulates viral gene expression by enhancing the export of partially spliced or nonspliced viral transcripts to the cytoplasm. This activity is mediated by the interaction of Rev with a highly structured viral RNA sequence, the Rev response element (RRE). In this paper, Rev regulation has been recapitulated in the yeast Saccharomyces cerevisiae. The data show that Rev enhances yeast pre-mRNA translation in an RRE-dependent manner and requires intact RNA binding and effector domains. In addition, reporter gene mutations in the 5' splice site and in the branch point are epistatic to and eliminate Rev responsiveness, i.e. some spliceosome assembly pathway steps precede a functional interaction with Rev. The results indicate that some of the Rev-interacting components are conserved between yeast and mammals and suggest that Rev acts on yeast pre-mRNA in the context of the spliceosome.

MeSH Terms
Biological Transport Cell Compartmentation DNA Mutational Analysis Gene Products, rev/genetics,metabolism Genes, Reporter HIV-1/genetics Metallothionein/genetics,metabolism Models, Genetic Protein Binding Protein Biosynthesis RNA Precursors/metabolism RNA Splicing Recombinant Fusion Proteins/metabolism Saccharomyces cerevisiae/genetics,metabolism rev Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, rev RNA Precursors Recombinant Fusion Proteins rev Gene Products, Human Immunodeficiency Virus Metallothionein
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stutz F
Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, MA 02254.
Rosbash M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-09-01
Pages
4096-104
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395332
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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