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PMID: 8108397 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A small element from the Mason-Pfizer monkey virus genome makes human immunodeficiency virus type 1 expression and replication Rev-independent.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 4 ·1994-02-15 ·Pages 1256-60

Bray M, Prasad S, Dubay JW, Hunter E, Jeang KT, Rekosh D, Hammarskjöld ML

Abstract

Replication of human immunodeficiency virus type 1 (HIV-1) is dependent on the viral Rev protein. This protein acts in concert with the cis-acting rev-responsive element present in intron-containing RNAs to facilitate nuclear export of these RNAs. Here we show that a cis-acting 219-nucleotide sequence from an unrelated "simple" retrovirus, Mason-Pfizer monkey virus (MPMV), enables Rev-independent HIV-1 replication. This sequence is present in an untranslated region near the 3' end of the MPMV genome. The MPMV element is also able to efficiently substitute for Rev in expression of Gag/Pol and Env proteins from subgenomic constructs. We hypothesize that the MPMV element functions by interacting with a cellular factor that plays a role in mRNA transport analogous to that of the Rev protein. It might be possible to exploit this element in the development of an HIV vaccine.

MeSH Terms
Biological Transport Cells, Cultured DNA Mutational Analysis Fusion Proteins, gag-pol/biosynthesis Gene Expression Gene Products, env/biosynthesis Gene Products, gag/biosynthesis Gene Products, rev/deficiency,genetics,metabolism Genome, Viral HIV-1/growth & development Mason-Pfizer monkey virus/genetics RNA, Messenger/metabolism RNA, Viral/metabolism Regulatory Sequences, Nucleic Acid/genetics Sequence Deletion Sequence Homology, Nucleic Acid Virus Replication rev Gene Products, Human Immunodeficiency Virus
Chemicals
Fusion Proteins, gag-pol Gene Products, env Gene Products, gag Gene Products, rev RNA, Messenger RNA, Viral rev Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Bray M
Myles H. Thaler Center for AIDS and Human Retrovirus Research, University of Virginia, Charlottesville 22908.
Prasad S
Dubay J W
Hunter E
Jeang K T
Rekosh D
Hammarskjöld M L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.4.1256
Published
1994-02-15
Pages
1256-60
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43136
Subset
IM
Grants
NIAID NIH HHS · AI25721 · United States
NIAID NIH HHS · AI25784 · United States
NIAID NIH HHS · AI27290 · United States
External Links
PubMed source DOI: 10.1073/pnas.91.4.1256 PMC Full Text View journal details
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