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PMID: 8146186 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning and functional expression of two monocyte chemoattractant protein 1 receptors reveals alternative splicing of the carboxyl-terminal tails.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 7 ·1994-03-29 ·Pages 2752-6

Charo IF, Myers SJ, Herman A, Franci C, Connolly AJ, Coughlin SR

Abstract

Monocyte chemoattractant protein 1 (MCP-1) is a member of the chemokine family of cytokines that mediate leukocyte chemotaxis. The potent and specific activation of monocytes by MCP-1 may mediate the monocytic infiltration of tissues in atherosclerosis and other inflammatory diseases. We have isolated cDNAs that encode two MCP-1-specific receptors with alternatively spliced carboxyl tails. Expression of the receptors in Xenopus oocytes conferred robust mobilization of intracellular calcium in response to nanomolar concentrations of MCP-1 but not to related chemokines. The MCP-1 receptors are most closely related to the receptor for the chemokines macrophage inflammatory protein 1 alpha and RANTES (regulated on activation, normal T expressed and secreted). The identification of the MCP-1 receptor and cloning of two distinct isoforms provide powerful tools for understanding the specificity and signaling mechanisms of this important chemokine.

MeSH Terms
Alternative Splicing Amino Acid Sequence Animals Calcium/metabolism Chemokine CCL2 Chemotactic Factors/metabolism Cloning, Molecular Humans Molecular Sequence Data Monocytes/physiology Oocytes/metabolism Receptors, CCR2 Receptors, Chemokine Receptors, Cytokine/biosynthesis,genetics Recombinant Proteins/biosynthesis Sequence Homology, Amino Acid Xenopus
Chemicals
CCR2 protein, human Chemokine CCL2 Chemotactic Factors Receptors, CCR2 Receptors, Chemokine Receptors, Cytokine Recombinant Proteins Calcium
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Charo I F
Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94141-9100.
Myers S J
Herman A
Franci C
Connolly A J
Coughlin S R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.7.2752
Published
1994-03-29
Pages
2752-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43448
Subset
IM
Grants
NHLBI NIH HHS · HL42662 · United States
NHLBI NIH HHS · HL43322 · United States
Databases
GENBANK
U03882, U03905
External Links
PubMed source DOI: 10.1073/pnas.91.7.2752 PMC Full Text View journal details
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