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PMID: 8156999 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Role of phospholipase C in Dictyostelium: formation of inositol 1,4,5-trisphosphate and normal development in cells lacking phospholipase C activity.

The EMBO journal ·Vol. 13 ·No. 7 ·1994-04-01 ·Pages 1601-9

Drayer AL, Van der Kaay J, Mayr GW, Van Haastert PJ

Abstract

The micro-organism Dictyostelium uses extracellular cAMP to induce chemotaxis and cell differentiation. Signals are transduced via surface receptors, which activate G proteins, to effector enzymes. The deduced protein sequence of Dictyostelium discoideum phosphatidylinositol-specific phospholipase C (PLC) shows strong homology with the mammalian PLC-delta isoforms. To study the role of PLC in Dictyostelium, a plc- mutant was constructed by disruption of the PLC gene. No basal or stimulated PLC activity could be measured during the whole developmental programme of the plc- cells. Loss of PLC activity did not result in a visible alteration of growth or development. Further analysis showed that developmental gene regulation, cAMP-mediated chemotaxis and activation of guanylyl and adenylyl cyclase were normal. Although the cells lack PLC activity, inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] was present at only slightly lower concentrations compared with control cells. Mass analysis of inositol phosphates demonstrated the presence of a broad spectrum of inositol phosphates in Dictyostelium, which was unaltered in the plc- mutant. Cell labelling experiments with [3H]inositol indicated that [3H]Ins(1,4,5)P3 was formed in a different manner in the mutant than in control cells.

MeSH Terms
Animals Cell Differentiation Chemotaxis/physiology Cyclic AMP/pharmacology Dictyostelium/cytology,drug effects,physiology Dose-Response Relationship, Drug Inositol 1,4,5-Trisphosphate/biosynthesis Inositol Phosphates/analysis Mutation Second Messenger Systems Signal Transduction Type C Phospholipases/physiology
Chemicals
Inositol Phosphates Inositol 1,4,5-Trisphosphate Cyclic AMP Type C Phospholipases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Drayer A L
Department of Biochemistry, University of Groningen, The Netherlands.
Van der Kaay J
Mayr G W
Van Haastert P J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-04-01
Pages
1601-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394990
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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