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PMID: 8163931 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

B cells are essential for murine mammary tumor virus transmission, but not for presentation of endogenous superantigens.

The Journal of experimental medicine ·Vol. 179 ·No. 5 ·1994-05-01 ·Pages 1457-66

Beutner U, Kraus E, Kitamura D, Rajewsky K, Huber BT

Abstract

Murine mammary tumor viruses (MMTVs) are retroviruses that encode superantigens capable of stimulating T cells via superantigen-reactive T cell receptor V beta chains. MMTVs are transmitted to the suckling offspring through milk. Here we show that B cell-deficient mice foster nursed by virus-secreting mice do not transfer infectious MMTVs to their offspring. No MMTV proviruses could be detected in the spleen and mammary tissue of these mice, and no deletion of MMTV superantigen-reactive T cells occurred. By contrast, T cell deletion and positive selection due to endogenous MMTV superantigens occurred in B cell-deficient mice. We conclude that B cells are essential for the completion of the viral life cycle in vivo, but that endogenous MMTV superantigens can be presented by cell types other than B cells.

MeSH Terms
Animals Antigen-Presenting Cells/immunology Antigens, Viral/immunology B-Lymphocytes/immunology Base Sequence Breast/microbiology DNA Female Mammary Tumor Virus, Mouse/immunology,physiology Mice Mice, Inbred AKR Mice, Inbred C57BL Molecular Sequence Data Proviruses/isolation & purification Retroviridae Infections/immunology,transmission Spleen/microbiology Superantigens/immunology T-Lymphocytes/immunology Virus Replication
Chemicals
Antigens, Viral Superantigens DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Beutner U
Program of Immunology, Sackler School of Graduate Biomedical Sciences, Tufts University, Boston, Massachusetts 02111.
Kraus E
Kitamura D
Rajewsky K
Huber B T
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47 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.179.5.1457
Published
1994-05-01
Pages
1457-66
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2191484
Subset
IM
Grants
NIAID NIH HHS · R01 AI-14910 · United States
External Links
PubMed source DOI: 10.1084/jem.179.5.1457 PMC Full Text View journal details
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