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PMID: 8188609 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

gyrB mutations in coumermycin A1-resistant Borrelia burgdorferi.

Journal of bacteriology ·Vol. 176 ·No. 10 ·1994-05-00 ·Pages 3072-5

Samuels DS, Marconi RT, Huang WM, Garon CF

Abstract

We have isolated and characterized mutants of Borrelia burgdorferi that are resistant to the antibiotic coumermycin A1, which targets the B subunit of DNA gyrase. Mutants had either 100- or 300-fold higher resistance to coumermycin A1 than wild-type B. burgdorferi. In each case, a single point mutation in the gyrB gene converted Arg-133 to Gly or Ile. Mutations in the homologous Arg residue of Escherichia coli DNA gyrase are also associated with resistance to coumarin antimicrobial agents.

Related Genes
gyrB
MeSH Terms
Amino Acid Sequence Aminocoumarins Anti-Bacterial Agents/pharmacology Base Sequence Borrelia burgdorferi Group/genetics Coumarins/pharmacology DNA Gyrase DNA Topoisomerases, Type II/genetics Drug Resistance, Microbial/genetics Molecular Sequence Data Point Mutation Sequence Homology, Amino Acid
Chemicals
Aminocoumarins Anti-Bacterial Agents Coumarins DNA Gyrase DNA Topoisomerases, Type II coumermycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Samuels D S
Laboratory of Vectors and Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840.
Marconi R T
Huang W M
Garon C F
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39 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.10.3072-3075.1994
Published
1994-05-00
Pages
3072-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205466
Subset
IM
Grants
NIGMS NIH HHS · GM21960 · United States
Databases
GENBANK
L14948, M38373, M58352, M59981, U00592, X02369, X04341, X53555, X54631, X71437
External Links
PubMed source DOI: 10.1128/jb.176.10.3072-3075.1994 PMC Full Text View journal details
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